Symptoms of Pepino mosaic virus in greenhouse tomatoes and reactions of test plants on infection

This study was carried out to detect the Pepino mosaic virus in various tomato hybrids grown in greenhouses. Total of 194 plant sample were collected from the greenhouse during 2019–2020 years. As a results of DAS-ELISA was found 54 of samples with Pepino mosaic virus, which was identified both in monoinfection and in the complex with other viruses: Cucumber mosaic virus, Tobacco mosaic virus, Tomato mosaic virus and Potato virus X. The possible symptoms of Pepino mosaic virus during the growing season of tomatoes include interveinal chlorosis, deformations, mosaic and yellow spots on leaves and also blotchy ripening fruits. The reaction of 10 plant species to the inoculation of Pepino mosaic virus was established. The results showed the greatest susceptibility of Nicotiana rustica L. and Datura stramonium L., where the maximum concentration of viral particles was detected 4 weeks after infection (OD: 0.952–1.013). The results presented in the article can be used to diagnose Pepino mosaic virus during the monitoring of greenhouse tomato plantations.