网织红细胞计数

Q4 Nursing

Nursing Critical Care Pub Date : 2020-02-07 DOI:10.32388/s5p8lj

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引用次数: 3

摘要

原理:使用活体染色(如新亚甲基蓝)，沉淀网状细胞内残留的核糖体RNA。将等量的染色剂加入edta抗凝血中，将稀释混合物孵育，并制备涂片。检查涂片以确定网状红细胞的数量。含有两个或两个以上蓝色染色物质颗粒的红细胞称为网织红细胞。网织红细胞的数量以红细胞总数的百分比表示。1

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Reticulocyte count

Principle Using a supravital stain (e.g., new methylene blue), residual ribosomal RNA within the reticulocytes is precipitated. An equal volume of stain is added to EDTA-anticoagulated blood, the dilution mixture incubated, and a smear is prepared. The smear is examined to determine the number of reticulocytes present. An erythrocyte containing two or more particles of blue-stained material is a reticulocyte. The number of reticulocytes is expressed as a percentage of the total number of erythrocytes counted. 1

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Nursing Critical Care Nursing-Critical Care Nursing

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