Study of Antibacterial Activity of Gentamicin-Cetirizine on Uropathogenic Escherichia coli Isolates

Article Type Original Research Authors Leila Hasani, MSc1 Leila Fozouni, PhD1* How to cite this article Hasani L., Fozouni L. Study of Antibacterial Activity of Gentamicin-Cetirizine on Uropathogenic Escherichia coli Isolates. Infection Epidemiology and Microbiology. 2021;7(1): 37-43 1 Department of Biology, Gorgan Branch, Islamic Azad University, Gorgan, Iran * Correspondence Address: Department of Biology, Gorgan Branch, Islamic Azad University, Gorgan, Iran. Tel:+989111518674 lili_kia@yahoo.com Article History Received: October 29 ,2020 Accepted: December 25 ,2020 Published: January 23 ,2021 Background: Urinary tract infections (UTIs) cause a wide range of infections in individuals; they are common nosocomial infections that have recently become difficult to treat because of the increased emergence of multidrug-resistant bacteria. The present study aimed to determine and compare the minimum inhibitory concentration of gentamicin alone and in combination with cetirizine against Escherichia coli strains isolated from hospitalized patients with UTI. Materials & Methods: This study was performed on 76 E. coli strains isolated from a total of 103 samples of patients admitted to three hospitals in Gonbad-e Kavus. Kirby Bauer disk diffusion and broth microdilution tests were used to determine antibiotic susceptibility and the minimum inhibitory concentration (MIC) of gentamicin alone and in combination with cetirizine according to CLSI M100-S25 (2015) criteria. Findings: Evaluation of the minimum inhibitory concentration of gentamicin-cetirizine combination against E. coli isolates showed that none were able to grow at a concentration of 8 μg/mL. The concentration of gentamicin in combination with cetirizine, inhibiting 90% of E. coli isolates (MIC90), was 4 μg/mL, which was 16 times lower than that of gentamicin alone (MIC90= 64 μg/mL) (p=.02). Conclusion: Gentamicin in combination with cetirizine was found to be more potent in inhibiting E. coli isolates than gentamicin alone. Therefore, the results of this study could provide a clear perspective for dealing with drug-resistant pathogens. Copyright© 2021, TMU Press. This open-access article is published under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License which permits Share (copy and redistribute the material in any medium or format) and Adapt (remix, transform, and build upon the material) under the Attribution-NonCommercial terms. 10.29252/iem.7.1.37