Genetic Diversity of Wild Lycoris radiata(Amaryllidaceae) from China Revealed by SRAP

Genetic diversity of 24 wild Lycoris radiata collected from different localities in China was examined by sequence-related amplified polymorphism(SRAP). Two hundred and eighteen loci were identified with 10 SRAP primer combinations,out of which 173 were polymorphic and accounted for 79.36% of total genetic diversity at species level. Observed number of alleles (na),effective number of alleles (ne),Nei's gene diversity (h) and Shannon information index (I) were 1.7936,1.4131,0.2415 and 0.3664,respectively. The coefficient of gene differentiation (Gst) and gene flow (Nm) were 0.9547 and 0.0136,suggesting that most of variation occurred among different resources of L.radiata,and the genetic showed significant differentiation. UPGMA cluster analysis of the 24 resources based on Nei's genetic distance showed two major clusters. Cluster I included 7 resources from southwest and northwest China,except for Jiangsu,Lianyungang resource(JS3). Cluster II included the rest 17 resources from south China to east China. The subgroups exhibited related to the morphology and habit of different resources of L.radiata to some extent. There was no significant correlation between genetic diversity and longitude,altitude,latitude,annual rainfall,and annual average temperature. The results suggested that the genetic diversity of wild L.radiata was high and the possible reasons for significant genetic differentiation might due to the very low gene flow. The results of this study might be useful for guiding the exploitation and conservation of germplasm of L.radiata.