利用y染色体STR标记评价印度中部Rewa人群遗传多态性

A. Mishra, U. Gondhali, S. Mishra, S. Choudhary
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摘要

该研究的目的是通过瞄准y短串联重复序列(STR)分析来自印度中部Rewa男性人群的不相关样本,然后将结果与先前发表的Y-STR单倍型数据进行比较。材料与方法使用AmpFlSTR®Yfiler™多重检测试剂盒(Thermo Fisher Scientific, USA)对来自Rewa人群的181名无亲和关系的男性受试者进行17种Y-STRs基因分型(DYS19、DYS389I、DYS389II、DYS390、DYS391、DYS392、DYS393、DYS385a/b、DYS437、DYS438、DYS439、DYS448、DYS456、DYS458、DYS635和Y-GATA-H4)。评估等位基因频率和法医学参数。结果共鉴定出111个不同的Y-STR等位基因,频率范围为0.006 ~ 0.829。DYS437基因多样性值为0.3092 ~ 0.8188。该群体具有较高的单倍型多样性(0.9985)和鉴别能力(0.927)。并进行了单倍型分析。在181个无亲缘关系的雄性样本中,共发现165个单倍型和153个独特单倍型。此外,利用分子方差分析(AMOVA)计算了研究群体和文献中描述的其他18个群体的Rst(遗传距离)值。Rst为利用微卫星数据估计遗传分化水平提供了一个方便的参数。基于这些Rst值,利用多维尺度图,构造了一棵邻居连接树。结论单倍型多样性高,辨别能力强,在种群中具有较大的雄性个体鉴别潜力。目前的人口数据有望在法医案件和人口遗传学中找到它们的用途。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Assessment of Genetic Polymorphisms in the Rewa Population of Central India Using Y-Chromosomal STR Markers
The aim of the study was to analyze unrelated samples from the Rewa male population of Central India by targeting Y-short tandem repeats (STR) and then compare the results to previously published Y-STR haplotype data. Materials and Methods A total of 181 unrelated male subjects from the Rewa population were genotyped for seventeen Y-STRs (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385a/b, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635, and Y-GATA-H4) by using an AmpFlSTR® Yfiler™ multiplex kit (Thermo Fisher Scientific, USA). The allele frequencies and forensic parameters were evaluated. Results A total of 111 distinct Y-STR alleles with corresponding frequencies ranging from 0.006 to 0.829 were identified. The gene diversity values ranged from 0.3092 at DYS437 to 0.8188 at DYS385b. The studied population showed a high level of haplotype diversity (0.9985) and discrimination capacity (0.927). A haplotype analysis was also conducted. Among the 181 unrelated male samples, 165 haplotypes and 153 unique haplotypes were found. Additionally, Rst (genetic distance) values were calculated using the analysis of molecular variance (AMOVA) for the studied population and for other 18 populations described in the literature. The Rst provides a convenient parameter for estimating the level of genetic differentiation from the microsatellite data. Based on these Rst values and using the multidimensional scaling plot, a neighbor-joining tree was constructed. Conclusion The high values of haplotype diversity and discrimination capacity indicate a great potential for distinguishing between male individuals in the studied population. The present population data are expected to find their use in forensic caseworks and population genetics.
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