两栖潮间带泥鳅鳃离子转运蛋白基因转录对高盐度的响应

IF 0.9 4区生物学 Q3 MARINE & FRESHWATER BIOLOGY

Marine and Freshwater Behaviour and Physiology Pub Date : 2020-07-03 DOI:10.1080/10236244.2020.1790301

Hamed Abdolahi-Fini, A. Akbarzadeh, I. Sourinejad

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引用次数: 0

摘要

摘要本研究探讨了高盐度红树林潮带水陆两栖泥鳉Na+/K+- atp酶α1 (NKAα1)和Na+/K+/2Cl−共转运体1a(NKCC1a)鳃mRNA的表达。鱼暴露在四种盐度下，分别为35(对照)、45、55和70 g kg - 1。在55和70 g kg - 1盐度条件下，NKAα1的鳃转录本升高，并在暴露中期(11天)后显著升高。短期(3天)和长期(21天)暴露后，NKCC1a基因在不同盐度组中的表达没有明显变化，但在55 g kg−1时，NKCC1a基因在中期出现强烈上调。NKAα1和NKCC1a的表达在长期暴露于盐度后恢复到基线水平，表明完全的分子和生理驯化。这些结果证明了离子转运酶在两栖鱼类适应高盐度环境中的渗透调节作用。

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Transcription of branchial ion transporter genes in response to high salinity in the amphibious, inter-tidal mudskipper Periophthalmus waltoni

ABSTRACT In this study, we explored the gill mRNA expression ofNa+/K+-ATPase α1 (NKAα1) and Na+/K+/2Cl− cotransporter 1a(NKCC1a) in an amphibious mudskipper species, Periophthalmus waltoniat high salinities of mangrove tidal zones. Fish were exposed to four salinities including 35 (control), 45, 55, and 70 g kg−1. The branchial transcripts of NKAα1 were elevated in fish kept at 55 and 70 g kg−1 salinities with a strong elevation after medium-term (11 days) of exposure. No obvious changes were observed in NKCC1a expression among different salinity groups after short-term (3 days) and long-term (21 days) of exposure, except for a medium-term strong upregulation of NKCC1agene at 55 g kg−1. The NKAα1 and NKCC1a expressions returned to baseline levels after the long term exposure salinity period, suggesting complete molecular and physiological acclimation.These results demonstrate the osmoregulatory role of ion transporter enzymes in acclimation of amphibious fish to high salinities.

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来源期刊

Marine and Freshwater Behaviour and Physiology 生物-海洋与淡水生物学

CiteScore

2.10

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Marine and Freshwater Behaviour and Physiology is devoted to the publication of papers covering field and laboratory research into all aspects of the behaviour and physiology of all marine and freshwater animals within the contexts of ecology, evolution and conservation. As the living resources of the world’s oceans, rivers and lakes are attracting increasing attention as food sources for humans and for their role in global ecology, the journal will also publish the results of research in the areas of fisheries biology and technology where the behaviour and physiology described have clear links to the contexts mentioned above. The journal will accept for publication Research Articles, Reviews, Rapid Communications and Technical Notes (see Instructions for authors for details). In addition, Editorials, Opinions and Book Reviews (invited and suggested) will also occasionally be published. Suggestions to the Editor-In-Chief for Special Issues are encouraged and will be considered on an ad hoc basis. With the goal of supporting early career researchers, the journal particularly invites submissions from graduate students and post-doctoral researchers. In addition to recognising the time constraints and logistical limitations their research often faces, and their particular need for a prompt review process, accepted articles by such researchers will be given prominence within the journal (see Instructions for authors for details).