{"title":"铬(VI)污染场所分离细菌的六价铬还原和16S rDNA鉴定","authors":"A. Das, Susmita Mishra","doi":"10.5580/10ae","DOIUrl":null,"url":null,"abstract":"A Gram-positive, hexavalent chromium [chromate: Cr (VI)]-resistant & reducing bacterium, isolated from sukind chromite mines, jajpur, India, was identified as a Brevibacterium casei(Gene Bank Accession Number: EU781952) by gene sequence homology. The strain(designated as APD15) could tolerate chromium up to a maximum concentration of 500 ppm, at optimum temperature and pH 30C and 7 for maximum chromium reduction. Agar supplemented with 100g Cr (VI)/ml as K2Cr2O7 and 0.5% (w/v) dextrose used as a carbon source. The results of the study indicated removal of more than 94% chromium (VI) by Brevibacterium caseidetermined by diphenylcarbazide colorimetric assay.","PeriodicalId":22514,"journal":{"name":"The Internet journal of microbiology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2008-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"7","resultStr":"{\"title\":\"Hexavalent chromium reduction and 16S rDNA identification of bacteria isolated from a Cr (VI) contaminated site\",\"authors\":\"A. Das, Susmita Mishra\",\"doi\":\"10.5580/10ae\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A Gram-positive, hexavalent chromium [chromate: Cr (VI)]-resistant & reducing bacterium, isolated from sukind chromite mines, jajpur, India, was identified as a Brevibacterium casei(Gene Bank Accession Number: EU781952) by gene sequence homology. The strain(designated as APD15) could tolerate chromium up to a maximum concentration of 500 ppm, at optimum temperature and pH 30C and 7 for maximum chromium reduction. Agar supplemented with 100g Cr (VI)/ml as K2Cr2O7 and 0.5% (w/v) dextrose used as a carbon source. The results of the study indicated removal of more than 94% chromium (VI) by Brevibacterium caseidetermined by diphenylcarbazide colorimetric assay.\",\"PeriodicalId\":22514,\"journal\":{\"name\":\"The Internet journal of microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2008-12-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"7\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Internet journal of microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5580/10ae\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Internet journal of microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5580/10ae","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Hexavalent chromium reduction and 16S rDNA identification of bacteria isolated from a Cr (VI) contaminated site
A Gram-positive, hexavalent chromium [chromate: Cr (VI)]-resistant & reducing bacterium, isolated from sukind chromite mines, jajpur, India, was identified as a Brevibacterium casei(Gene Bank Accession Number: EU781952) by gene sequence homology. The strain(designated as APD15) could tolerate chromium up to a maximum concentration of 500 ppm, at optimum temperature and pH 30C and 7 for maximum chromium reduction. Agar supplemented with 100g Cr (VI)/ml as K2Cr2O7 and 0.5% (w/v) dextrose used as a carbon source. The results of the study indicated removal of more than 94% chromium (VI) by Brevibacterium caseidetermined by diphenylcarbazide colorimetric assay.
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