高效液相色谱法测定龙葵中核黄素的含量

B. Varynskyi, M. Y. Zeleniuk, A. Kaplaushenko
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摘要

目的:建立快速、选择性、可重复性高的高效液相色谱法测定多种维生素制剂“Revit”中核黄素含量的方法。材料和方法。脱气器,二元泵,自动进样器,恒温柱室,二极管阵列检测器。色谱柱Zorbax SB-C18, 30 mm × 4.6 mm, 1.8µm。样品采用Kern ABT 100-5M分析电子秤,超声波浴液超声波XUBA 3,尼龙超滤0.12µm制备。在洗脱液中选用0.1%甲酸作为缓冲溶液。在pH为2 ~ 4时,观察核黄素的logD最大值。因此,使用洗液时观察到核黄素的最大保留,рН对应于此间隔。0.1%甲酸溶液的pH值为2.7。因此,我们选择了这种缓冲液作为洗脱液的一部分。选择乙腈作为有机改性剂。以10%乙腈为最佳含量。在化学分析性质的基础上,研究了核黄素的色谱行为。确定了色谱测定的最佳条件,建立了测定“Revit”糖蜜中核黄素含量的快速、特异的方法。该技术在一个真实的样品“Revit”dragee阐述。该方法推荐用于控制实验室、分析实验室和研究实验室。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Determination of riboflavin in dragee “Revit” by HPLC
The aim of the work is development of express, selective, reproducible methods for the determination of riboflavin by HPLC in a multivitamin preparation, dragee “Revit”. Materials and methods. Degasser, binary pump, autosampler, thermostated column compartment, diode array detector. Chromatographic column Zorbax SB-C18, 30 mm × 4.6 mm, 1.8 µm. Samples were prepared using analytical electronic scales Kern ABT 100-5M, ultrasonic bath Ultrasonic XUBA 3, nylon ultrafilters 0.12 µm. Results. 0.1 % formic acid was selected as a buffer solution in the eluent. The maximum values of logD were observed at intervals for riboflavin at pH from 2 to 4. Therefore, the maximum retention of the riboflavin was observed when using the eluent, рН which corresponds to this interval. The pH value for 0.1 % formic acid solution was 2.7. Therefore, such a buffer was chosen by us as part of the eluent. Acetonitrile was selected as the organic modifier. The optimal content of the compound was observed at 10 % acetonitrile. Conclusions. The chromatographic behavior of riboflavin on the basis of chemical-analytical properties was studied. The optimal conditions for chromatographic determination were determined and a specific, express method for the determination of riboflavin in “Revit” dragee was developed. The technique on a real sample of “Revit” dragee was elaborated. The method was recommended for use in control and analytical and research laboratories.  
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