茶花产品中表没食子儿茶素-3-没食子酸酯(EGCG)含量的筛选

Nurul Ain Syukriyah Ahmad Muhamud, S. Amran
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引用次数: 1

摘要

流行病学研究证实了饮茶对预防慢性疾病的影响。茶(Camellia sinensis)是表没食子儿茶素-3-没食子酸酯(EGCG)化合物的主要来源,具有抗肥胖、抗糖尿病、抗炎症等药理特性。本研究的目的是分析和比较当地市场上的商业化茶基产品、冲泡茶和即饮茶饮料中EGCG的含量。本研究分为两个阶段。第一阶段涉及从四种茶叶中提取EGCG化合物,即白茶(WT),绿茶(GT),乌龙茶(OT)和红茶(BT),采用水萃取和甲醇萃取技术。采用高效液相色谱法测定EGCG的含量。第二阶段采用高效液相色谱法对泡茶包(ITB)和即饮茶饮料(RTD)中的EGCG化合物进行表征。我们的研究结果表明,与使用甲醇和水萃取方法的其他茶叶类型相比,GT含有最高的EGCG浓度。温度和萃取时间对萃取率有影响。这项研究还表明,与冲泡茶相比,即饮茶饮料中EGCG的含量明显较低,因此,即饮饮料不太健康,尤其是对肥胖和糖尿病患者而言。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Screening of Epigallocatechin-3-Gallate (EGCG) Content in Camellia Sinensis Products
Epidemiological studies have proven the influences of tea consumption in prevention of chronic diseases. Tea (Camellia sinensis) is a major source of epigallocatechin-3-gallate (EGCG) compound with pharmacological properties such as anti-obesity, anti-diabetes, anti-inflammation. The aims of this study is to analyze and compare the EGCG content in commercialized tea-based products, infusion tea and ready-to-drink tea beverages available in the local market. This study is divided into two phases. The first phase involved the extraction of EGCG compound from four types of tea namely as white tea (WT), green tea (GT), oolong tea (OT) and black tea (BT) using aqueous and methanol extraction techniques. The amount of EGCG is determined using HPLC. The second phase involved the characterization of EGCG compound in infusion tea bags (ITB) and ready-to-drink tea beverages (RTD) using HPLC. Our findings recorded that GT contain the highest EGCG concentration in comparison to other tea types using both methanol and aqueous extraction methods. Temperature and extraction time have influence on the extraction yield. This study also revealed that ready-to-drink tea beverages contains significantly lower level of EGCG compared to the infusion tea hence makes ready-to-drink beverages less healthy for consumption especially for obese and diabetics individuals.
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