{"title":"家蚕消化液35K蛋白酶的遗传变异与遗传","authors":"Yonghuang Jiang, Y. Banno, H. Fujii","doi":"10.11416/KONTYUSHIGEN1930.69.225","DOIUrl":null,"url":null,"abstract":"The digestive juice of the larvae of the silkworm, Bombyx mori include many proteases. Some were isolated and characterized (EGUCHI and IWAMOTo, 1982; SASAKI and SUZUKI, 1982; KIDO et al., 1986; KoTANI et al., 1999), but their heterogeneity has not been investigated, for except one case (EGUCHI and YOSHITAKE, 1967). We purified an enzyme named as 35K protease from silkworm digestive juice (JIANG et al., 2000). This enzyme is a chymotrypsin-like protease and was inhibited by CI-8 (SHIRAI et al., 1997) in the larval hemolymph. During our investigation, we discovered polymorphism of the 35K protease from digestive juice of silkworm. In this paper, we focus on polymorphism and the mode of inheritance of 35K protease.","PeriodicalId":22665,"journal":{"name":"The journal of sericultural science of Japan","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2000-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Genetic variant and inheritance of the 35K protease in digestive juice of silkworm, Bombyx mori\",\"authors\":\"Yonghuang Jiang, Y. Banno, H. Fujii\",\"doi\":\"10.11416/KONTYUSHIGEN1930.69.225\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The digestive juice of the larvae of the silkworm, Bombyx mori include many proteases. Some were isolated and characterized (EGUCHI and IWAMOTo, 1982; SASAKI and SUZUKI, 1982; KIDO et al., 1986; KoTANI et al., 1999), but their heterogeneity has not been investigated, for except one case (EGUCHI and YOSHITAKE, 1967). We purified an enzyme named as 35K protease from silkworm digestive juice (JIANG et al., 2000). This enzyme is a chymotrypsin-like protease and was inhibited by CI-8 (SHIRAI et al., 1997) in the larval hemolymph. During our investigation, we discovered polymorphism of the 35K protease from digestive juice of silkworm. In this paper, we focus on polymorphism and the mode of inheritance of 35K protease.\",\"PeriodicalId\":22665,\"journal\":{\"name\":\"The journal of sericultural science of Japan\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2000-06-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The journal of sericultural science of Japan\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.11416/KONTYUSHIGEN1930.69.225\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The journal of sericultural science of Japan","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.11416/KONTYUSHIGEN1930.69.225","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
摘要
家蚕(Bombyx mori)幼虫的消化液含有许多蛋白酶。有些被分离出来并被鉴定(EGUCHI和IWAMOTo, 1982;SASAKI and SUZUKI, 1982;KIDO et al., 1986;KoTANI et al., 1999),但除了一个案例(EGUCHI and YOSHITAKE, 1967)外,他们的异质性尚未被调查。我们从蚕的消化液中纯化了一种酶,命名为35K蛋白酶(JIANG等,2000)。这种酶是一种凝乳胰蛋白酶样蛋白酶,在幼虫血淋巴中被CI-8抑制(SHIRAI et al., 1997)。在我们的调查中,我们发现了蚕消化液中35K蛋白酶的多态性。本文主要对35K蛋白酶的多态性及其遗传方式进行了研究。
Genetic variant and inheritance of the 35K protease in digestive juice of silkworm, Bombyx mori
The digestive juice of the larvae of the silkworm, Bombyx mori include many proteases. Some were isolated and characterized (EGUCHI and IWAMOTo, 1982; SASAKI and SUZUKI, 1982; KIDO et al., 1986; KoTANI et al., 1999), but their heterogeneity has not been investigated, for except one case (EGUCHI and YOSHITAKE, 1967). We purified an enzyme named as 35K protease from silkworm digestive juice (JIANG et al., 2000). This enzyme is a chymotrypsin-like protease and was inhibited by CI-8 (SHIRAI et al., 1997) in the larval hemolymph. During our investigation, we discovered polymorphism of the 35K protease from digestive juice of silkworm. In this paper, we focus on polymorphism and the mode of inheritance of 35K protease.