Effect of Persistent Hyperglycemia and Lipid Profile and its role in Glycation and Oxidative stress in Chronic Type 2 Diabetes Mellitus subjects

Introduction: Diabetes mellitus is a disease of carbohydrate metabolism disorder which results in increase in blood glucose level. Hyperglycemia may increase oxidative stress through a variety of ways impairs an antioxidant defenses mechanism. Reactive oxygen species are increased as a result of the oxidative stress caused by hyperglycemia, which activates a number of redox-sensitive cell signaling molecules producing cytotoxic materials. Methods: 250 randomly visited the OPD at the Chitwan Medical College Department of Medicine were chosen for this cross-sectional study. 10ml of blood sample was drawn from anticubital vein following overnight fasting and was centrifuged at room temperature for 10 minutes at 3000 rpm. The serum was stored at 4oC for biochemical investigations. Fasting blood sugar, lipid profile and HbA1c etc. were analyzed via chemiluminescence Analyzer (CLIA) in the Hospital Laboratory of Chitwan Medical College. With SPSS version 22, statistical analysis was carried out. Results: IIt was found from the study that type 2 diabetic subjects have significant increase of fasting blood sugar and malondialdehyde (MDA) but glutathione peroxidase was found to be decreased in type 2 diabetic subjects as compared to healthy controls. Glutathione reductase was found non-significant at (P>0.05) in both the subjects. It was found from the study that association of HbA1c with Total cholesterol, Triglyceride, LDL-C was found to be positively correlated and was significant at P<0.05. Conclusions: When predicting glycemic control in T2DM patients, lipid profiles (LDL-C) and glycation status can be helpful tools.