Yoko Ino, H. Kagawa, Tomoko Akiyama, Yusuke Nakai, Sakura Ito, M. Shimoda, Makiko Kawamura, H. Hirano, Y. Kimura
{"title":"使用SAINOME-plate进行蛋白质组学的蛋白质分离","authors":"Yoko Ino, H. Kagawa, Tomoko Akiyama, Yusuke Nakai, Sakura Ito, M. Shimoda, Makiko Kawamura, H. Hirano, Y. Kimura","doi":"10.2198/JELECTROPH.62.11","DOIUrl":null,"url":null,"abstract":"SUMMARY The SAINOME-plate consists of a 384-well plate and a cover that contains a cutter, which can cut a polyacryl-amide gel into approximately 4.5-mm square pieces following electrophoresis. In this study, we applied SDS-PAGE and the SAINOME-plate to fractionation of protein mixtures from cell extracts or serum for proteomic approaches. Compared with gel-fractionation using a cutter or a scalpel, SAINOME-plate gel-fractionation is simpler and higher-throughput. In terms of reproducibility of proteomic profiling, SAINOME-plate gel-fractionation was comparable to scalpel gel-fractionation. Additionally, human keratin contamination was lower with the SAINOME-plate than with a scalpel. In serum protein fractionation, the number of proteins identified increased approximately 2-fold and 3.7-fold relative to non-fractionation when the gel was divided into 8 and 96 fractions, respectively. The results demonstrate that the SAINOME-plate gel-fractionation will be a useful method in mass spectrometry-based proteomics.","PeriodicalId":15059,"journal":{"name":"Journal of capillary electrophoresis","volume":"93 1","pages":"11-15"},"PeriodicalIF":0.0000,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Protein fractionation for proteomics using the SAINOME-plate\",\"authors\":\"Yoko Ino, H. Kagawa, Tomoko Akiyama, Yusuke Nakai, Sakura Ito, M. Shimoda, Makiko Kawamura, H. Hirano, Y. Kimura\",\"doi\":\"10.2198/JELECTROPH.62.11\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"SUMMARY The SAINOME-plate consists of a 384-well plate and a cover that contains a cutter, which can cut a polyacryl-amide gel into approximately 4.5-mm square pieces following electrophoresis. In this study, we applied SDS-PAGE and the SAINOME-plate to fractionation of protein mixtures from cell extracts or serum for proteomic approaches. Compared with gel-fractionation using a cutter or a scalpel, SAINOME-plate gel-fractionation is simpler and higher-throughput. In terms of reproducibility of proteomic profiling, SAINOME-plate gel-fractionation was comparable to scalpel gel-fractionation. Additionally, human keratin contamination was lower with the SAINOME-plate than with a scalpel. In serum protein fractionation, the number of proteins identified increased approximately 2-fold and 3.7-fold relative to non-fractionation when the gel was divided into 8 and 96 fractions, respectively. The results demonstrate that the SAINOME-plate gel-fractionation will be a useful method in mass spectrometry-based proteomics.\",\"PeriodicalId\":15059,\"journal\":{\"name\":\"Journal of capillary electrophoresis\",\"volume\":\"93 1\",\"pages\":\"11-15\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of capillary electrophoresis\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2198/JELECTROPH.62.11\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of capillary electrophoresis","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2198/JELECTROPH.62.11","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Protein fractionation for proteomics using the SAINOME-plate
SUMMARY The SAINOME-plate consists of a 384-well plate and a cover that contains a cutter, which can cut a polyacryl-amide gel into approximately 4.5-mm square pieces following electrophoresis. In this study, we applied SDS-PAGE and the SAINOME-plate to fractionation of protein mixtures from cell extracts or serum for proteomic approaches. Compared with gel-fractionation using a cutter or a scalpel, SAINOME-plate gel-fractionation is simpler and higher-throughput. In terms of reproducibility of proteomic profiling, SAINOME-plate gel-fractionation was comparable to scalpel gel-fractionation. Additionally, human keratin contamination was lower with the SAINOME-plate than with a scalpel. In serum protein fractionation, the number of proteins identified increased approximately 2-fold and 3.7-fold relative to non-fractionation when the gel was divided into 8 and 96 fractions, respectively. The results demonstrate that the SAINOME-plate gel-fractionation will be a useful method in mass spectrometry-based proteomics.