金叶提取物合成金纳米颗粒体外抗菌及罗丹明B染料光催化降解活性研究

David Shine, S. Shasha, Alorku Kingdom, Okoampah Emmanuel, S. S. Fometu, Wu Guohua
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引用次数: 1

摘要

使用物理和化学方法合成纳米粒子的毒性和成本导致科学家们采用生物技术的出现。因此,本研究采用了一种生态友好的方法,即利用枇杷叶提取物作为氯金(HAuCl4)到金纳米颗粒(AuNPs)的还原剂和稳定剂。采用实时直接分析(DART-MS)进行植物化学筛选。然而,在抗菌和光催化研究之前,通过紫外可见光谱(UV/Vis),透射电子显微镜(TEM),能量色散x射线光谱,傅立叶透射红外光谱和x射线衍射光谱对AuNPs进行了表征。DART-MS显示了20多种植物化学物质,包括酚类化合物。傅里叶变换红外光谱显示了类黄酮的存在,我们假设这些类黄酮负责氯化金的还原反应。紫外/可见光谱在530 nm处显示出等离子体表面共振,透射电镜显示AuNPs为单分散,形状为球形和三角形,尺寸在3.4 ~ 39.7 nm之间。XRD也记录了面心立方AuNPs,平均晶粒尺寸为20.94 nm。所合成的AuNPs对革兰氏阳性菌金黄色葡萄球菌、粪肠球菌和革兰氏阴性菌大肠杆菌、铜绿假单胞菌均有抗菌作用。在UV/Vis照射下,AuNPs的光催化活性在75分钟内对罗丹明b (rhodamine b, RhB)有明显的降解作用。由于其无毒性,植物介导的生物合成AuNPs在加强其生物医学应用和废水处理方面具有重要价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Biosynthesizing Gold Nanoparticles with Parkia biglobosa Leaf Extract for Antibacterial Efficacy In Vitro and Photocatalytic Degradation Activities of Rhodamine B Dye
The toxicity and cost involved in the synthesis of nanoparticles using physical and chemical methods have led to the emergence of biological techniques being employed by scientists. Hence the present study employed an eco-friendly method by using Parkia biglobosa leaves extract as a reducing and stabilizing agent of chloroauric gold (HAuCl4) to gold nanoparticle (AuNPs). Direct Analysis in Real-Time (DART-MS) was employed for phytochemical screening. However, the AuNPs were characterized by Ultraviolet-Visible spectroscopy (UV/Vis), Transmission Electron Microscopy (TEM), Energy Dispersive X-ray spectroscopy, Fourier Transmission Infrared Spectroscopy, and X-ray diffraction spectroscopy, before the antibacterial and photocatalysis study. DART-MS showed more than 20 phytochemicals, including phenolic compounds. The FT-IR revealed the existence of flavonoids that we hypothesized are responsible for gold chloride reduction reactions. The optical properties of the gold nanoparticles by UV/Vis spectra registered plasmon surface resonance at a peak of 530 nm, whiles TEM revealed the AuNPs to be mono-dispersed and shapes were spherical and triangular with sizes ranging from 3.4 nm to 39.7 nm. XRD also recorded face-centered cubic AuNPs with 20.94 nm as the average crystallite size. The synthesized AuNPs were able to elucidate their anti-bacterial efficacy on Gram-positive bacteria Staphylococcus aureus, enterococcus faecalis and Gram-negative bacteria Escherichia coli, pseudomonas aeruginosa. The photocatalytic activities of AuNPs under UV/Vis irradiation exhibited a substantial degradation effect on rhodamine b (RhB) within 75 minutes. Due to their non-toxic nature, plant-mediated biosynthesized AuNPs, would be valuable in enhancing their biomedical applications and in wastewater treatment.
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