影响北卡州烟草作物的茄灰菌种群多样性

中国烟草科学 Pub Date : 2016-05-02 DOI:10.3381/15-047

M. Katawczik, H. Tseng, A. Mila

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引用次数: 5

摘要

对2007年和2008年从美国北卡罗来纳州11个烟草田分离到的184株茄枯菌进行了基因型多样性和侵袭性评价。所有菌株均为1号小种，生物变种1，属II种型。使用基于重复序列的聚合酶链反应评估菌株的遗传多样性。DNA引物(REP, ERIC, BOX)生成基因组指纹。REP和BOX均显示3种模式:Ar、Cr、Dr和Ab、Cb、Db。ERIC-PCR鉴定出5种模式。在80%的菌株中发现Ae型。Be型占4%，Ce型占13%，De型占2%，Ee型占1%。聚类分析表明，菌株相似度为88%，构成了一个相当均匀的群体。对3个不同抗青枯病水平的烟草品种进行了菌株侵袭性评价。总的来说，侵略性取决于菌株来自哪个领域……

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DIVERSITY OF RALSTONIA SOLANACEARUM POPULATIONS AFFECTING TOBACCO CROPS IN NORTH CAROLINA

One hundred eighty four strains of Ralstonia solanacearum isolated in 2007 and 2008 from 11 tobacco fields in North Carolina were evaluated for genotypic diversity and aggressiveness. All strains were race 1, biovar 1, and belonged to phylotype II. Genetic diversity of the strains was assessed with the use of repetitive sequence–based polymerase chain reaction. DNA primers (REP, ERIC, and BOX) were used to generate genomic fingerprints. Both REP and BOX revealed 3 patterns: Ar, Cr, and Dr and Ab, Cb, and Db, respectively. Five patterns were identified with ERIC-PCR. Pattern Ae was found in 80% of the strains collected. Pattern Be was in 4%, pattern Ce in 13%, pattern De in 2%, and pattern Ee in 1% of the strains collected. Cluster analyses showed that the strains were 88% similar and constitute a rather homogeneous group. Aggressiveness of strains was evaluated on 3 tobacco cultivars with different levels of resistance to bacterial wilt. Overall, aggressiveness depended on the field from which the strains...

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来源期刊

中国烟草科学烟草

自引率

0.00%

发文量

3722

期刊介绍： Chinese Tobacco Science is an academic scientific journal (bimonthly) under the supervision of the Ministry of Agriculture and Rural Affairs of the People's Republic of China, and sponsored by the Tobacco Research Institute of the Chinese Academy of Agricultural Sciences and the Qingzhou Tobacco Research Institute of China National Tobacco Corporation. It was founded in 1979 and is publicly distributed nationwide. The journal mainly publishes academic papers on scientific research results, new production technologies, and modern management in my country's tobacco science research and tobacco production technology. In addition, it also publishes forward-looking review articles in the field of tobacco research. There are columns such as tobacco genetics and breeding, cultivation technology, modulation and processing, physiology and biochemistry, plant protection, review or monograph, quality chemistry, etc.