人磷脂酰胆碱转移蛋白:纯化、结晶及初步x射线衍射数据

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology Pub Date : 2002-04-01 DOI:10.1016/S0167-4838(01)00318-1

Wayne W. Chan , Steven L. Roderick , David E. Cohen

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引用次数: 2

摘要

我们表达、纯化和结晶了重组人磷脂酰胆碱转移蛋白(PC-TP)和与二烯油基磷脂酰胆碱结合的硒代甲硫基PC-TP。硒代甲硫基PC-TP和天然PC-TP的生化性质没有区别，两种蛋白在相似的条件下结晶。原生和硒代甲硫基PC-TP在两个不同的空间群中结晶，并以2.4 Å分辨率衍射x射线。

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Human phosphatidylcholine transfer protein: purification, crystallization and preliminary X-ray diffraction data

We have expressed, purified and crystallized recombinant human phosphatidylcholine transfer protein (PC-TP) and selenomethionyl PC-TP bound to dilinoleoyl phosphatidylcholine. The biochemical properties of native and selenomethionyl PC-TP were indistinguishable, and the two proteins crystallized under similar conditions. Both native and selenomethionyl PC-TP crystallized in two distinct space groups and diffracted X-rays to 2.4 Å resolution.

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Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology

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