Optimum cultivation conditions for mass production of antagonistic bacterium Alcaligenes sp. HC12 effective in antagonistic of browning disease caused by Pseudomonas agarici

: This study was conducted to investigate optimum conditions for mass production of ntagonistic microbes Alcaligenes sp. HC12. Alcaligenes sp. HC12 had a potent biological control agent to control browning disease caused by Pseudomonas agarici. Alcaligenes sp. HC12 markedly showed the antagonistic activity against Pseudomonas agarici, the most destructive pathogen of cultivated mushrooms. To define the optimum conditions for the mass production of the Alcaligenes sp. HC12, we have investigated optimum culture conditions and effects of various nutrient source on the bacterial growth. The optimum initial pH and temperature were determined as pH 9.0 and 30 o , respectively. The optimal concentration of medium elements for the growth of pathogen inhibitor bacterium( Alcaligenes sp. HC12) was determined as follows: 0.5% dextrine, 1.5% yest extract, 1.0% NaNO 3 , 0.5% KH 2 PO 4 , and 1.5% asparagine.