山楂叶提取物绿色合成纳米银及其抗菌活性研究

N. Palei
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引用次数: 6

摘要

前言:以山药叶提取物为还原剂合成硝酸银纳米颗粒(AgNPs),并对其抗菌活性进行了评价。材料与方法:山茱萸提取物兼有还原剂和封盖剂的作用。傅里叶变换红外(FT-IR)研究鉴定了植物提取物中还原银离子(官能团)的化合物。用于表征合成纳米颗粒的各种技术是动态光散射、透射电子显微镜(TEM)、能量衍射x射线和紫外可见分光光度计来表征合成的AgNPs。结果和讨论:从FTIR研究中,证实存在一些官能团覆盖AgNPs。紫外可见分光光度计在436 ~ 446 nm范围内发现吸光度峰。所得AgNPs的平均粒径为86 nm。此外,制备的AgNPs对革兰氏阳性菌和革兰氏阴性菌的抑菌活性均高于普通提取物。结论:从实验结果可以看出,从金针菇提取物中绿色合成AgNPs具有简单、快速、一步法、生态友好、可替代常规方法的特点。从camara camara叶提取物合成的AgNP具有作为广谱抗菌药物的应用潜力。
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Green synthesis of silver nanoparticles using leaf extract of Lantana camara and its antimicrobial activity
Introduction: To synthesize silver nitrate nanoparticles (AgNPs) using aqueous leaf extracts of Lantana camara as a reducing agent and evaluates its antimicrobial activity. Materials and Methods: L. camara extract acts both reducing agents as well as capping agent. Fourier-transform infrared (FT-IR) study was used for identifying the compounds responsible for the reduction of silver ions, the functional groups present in the plant extract. Various techniques used to characterize synthesized nanoparticles are dynamic light scattering, transmission electron microscopy (TEM), energy diffraction X-ray, and ultraviolet–visible (UV Vis) spectrophotometer were performed to characterize synthesized AgNPs. Results and Discussion: From the FTIR study, it was confirmed the presence of some functional groups capping the AgNPs. UV-Vis spectrophotometer showed an absorbance peak in the range of 436–446 nm. The average particle size of the prepared AgNPs was found to be 86 nm. In addition, the antimicrobial activity of prepared AgNPs was found to be higher than plain extract using various Gram-positive and Gram-negative bacteria. Conclusions: From the results, it can be concluded that green synthesis of AgNPs from L. camera extract is easy, fast, one step, eco-friendly, and alternative conventional methods. The synthesized AgNP preparations from L. camara leaf extract show potential for application as broad-spectrum antimicrobial agents.
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