{"title":"逆转录-聚合酶链反应检测HGVGB-C型肝炎病毒RNA中5 ' -非翻译区","authors":"Yasuyuki Okamoto , Hiroshi Nakano , Masahide Yoshikawa , Eiryo Kikuchi , Hiroshi Fukui , Hirotsugu Okuda , Taikou Tamagawa , Yoshinori Matsuyama , Shoji Samma","doi":"10.1016/S0928-4346(96)00340-4","DOIUrl":null,"url":null,"abstract":"<div><p>Using newly-designed primers for semi-nested reverse transcriptase-polymerase chain reaction to detect 5′-untranslated region of <span><math><mtext>HGV</mtext><mtext>GB-C</mtext></math></span> hepatitis virus RNA, we screened 45 patients and found three <span><math><mtext>HGV</mtext><mtext>GB-C</mtext></math></span> positive patients (with fulminant hepatitis and liver cirrhosis, and on hemodialysis). Restriction fragment length polymorphism and single-strand conformation polymorphism analysis revealed a similar pattern in all positive specimens. The sequence amplified by our primers might be a highly conserved sequence. We believe our primers are useful for screening of <span><math><mtext>HGV</mtext><mtext>GB-C</mtext></math></span> virus RNA.</p></div>","PeriodicalId":13746,"journal":{"name":"International Hepatology Communications","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1997-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0928-4346(96)00340-4","citationCount":"1","resultStr":"{\"title\":\"Detection of the 5′-untranslated region in HGVGB-C hepatitis virus RNA by reverse transcriptase-polymerase chain reaction\",\"authors\":\"Yasuyuki Okamoto , Hiroshi Nakano , Masahide Yoshikawa , Eiryo Kikuchi , Hiroshi Fukui , Hirotsugu Okuda , Taikou Tamagawa , Yoshinori Matsuyama , Shoji Samma\",\"doi\":\"10.1016/S0928-4346(96)00340-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Using newly-designed primers for semi-nested reverse transcriptase-polymerase chain reaction to detect 5′-untranslated region of <span><math><mtext>HGV</mtext><mtext>GB-C</mtext></math></span> hepatitis virus RNA, we screened 45 patients and found three <span><math><mtext>HGV</mtext><mtext>GB-C</mtext></math></span> positive patients (with fulminant hepatitis and liver cirrhosis, and on hemodialysis). Restriction fragment length polymorphism and single-strand conformation polymorphism analysis revealed a similar pattern in all positive specimens. The sequence amplified by our primers might be a highly conserved sequence. We believe our primers are useful for screening of <span><math><mtext>HGV</mtext><mtext>GB-C</mtext></math></span> virus RNA.</p></div>\",\"PeriodicalId\":13746,\"journal\":{\"name\":\"International Hepatology Communications\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1997-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0928-4346(96)00340-4\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Hepatology Communications\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0928434696003404\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Hepatology Communications","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0928434696003404","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Detection of the 5′-untranslated region in HGVGB-C hepatitis virus RNA by reverse transcriptase-polymerase chain reaction
Using newly-designed primers for semi-nested reverse transcriptase-polymerase chain reaction to detect 5′-untranslated region of hepatitis virus RNA, we screened 45 patients and found three positive patients (with fulminant hepatitis and liver cirrhosis, and on hemodialysis). Restriction fragment length polymorphism and single-strand conformation polymorphism analysis revealed a similar pattern in all positive specimens. The sequence amplified by our primers might be a highly conserved sequence. We believe our primers are useful for screening of virus RNA.
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