一种新的异叶乌头环烯醇对Vero和肺细胞具有生物活性和细胞毒性

Md. Saiful Alam, S. Rony, F. Afroz, Abdullah Al Mansur, S. Sharmin, F. Moni, S. Akhter, M. M. Hussain, M. Sohrab
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摘要

本文对乌头进行了较为详细的植物化学和生物学研究。利用凝胶层析液相色谱(VLC)分离得到2个化合物1,1,8,8-三甲基-4-亚甲基-十氢- 1h -环戊-[8]-环烯-1-醇(1)和β-豆甾醇(2)。通过1D和2D NMR数据对化合物进行了表征。化合物1为首次从该植物中分离得到。采用圆盘扩散法筛选粗提物对几种革兰氏阳性和革兰氏阴性细菌和真菌的抑菌活性,观察到明显的抑菌活性。该提取物还使用盐水虾致死性生物测定法进行了细胞毒性测试,与标准抗癌药物硫酸长春新碱相比,其活性中等。通过对DPPH(2,2-二苯基-1-苦味基肼基)自由基的清除作用测定了其抗氧化性能,与标准抗氧化剂相比,其抗氧化活性中等(IC50 = 53.25 μg/mL)。采用高效液相色谱法测定粗提物中总酚、类黄酮含量和多酚类化合物含量。分离得到的化合物1和2对肺A549细胞具有剂量依赖性的抗增殖活性。达卡大学药学院。自然科学22(1):1- 10,2023 (6)
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A New Annulenol from Aconitum heterophyllum with Bioactivity and Cytotoxicity against Vero and Lung Cell Line
In this research work, a detailed phytochemical and biological investigation of Aconitum heterophyllum was accomplished. The cold methanolic root extract of A. heterophyllum was subjected to VLC for fractionation over silica gel, which led to the isolation of two compounds 1,1,8,8-trimethyl-4-methylene-decahydro- 1H-cyclopenta-[8]-annulen-1-ol (1) and β-stigmasterol (2). The compounds were characterized by analysis of their 1D and 2D NMR spectral data. Compound 1 is first reported from this plant. The crude extract was screened for antimicrobial activity against several gram-positive and gram-negative bacteria, and fungi using disk diffusion method where significant inhibitory activity was observed. The extract was also tested for cytotoxic property using brine shrimp lethality bioassay, which revealed moderate activity in comparison with the standard anticancer drug, vincristine sulphate. Antioxidant property of the crude methanol extract of A. heterophyllum was determined by the scavenging of the DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical and found moderate antioxidant activity (IC50 = 53.25 μg/mL) when compared to standard antioxidant. Total phenolic and flavonoid content and polyphenolic compound content of the crude extract were determined by HPLC. The isolated compounds 1 and 2 showed dosedependent antiproliferation activity against the vero and lung A549 cells. Dhaka Univ. J. Pharm. Sci. 22(1): 1-10, 2023 (June)
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