Challenges and opportunities of using immobilized lipase as biosensor

ABSTRACT Over the years, the science of biosensors has evolved significantly. The first or earliest generation of biosensors only detected either the decrease or increase of product or reactant-based natural mediators as the pathway for electron transfer. The subsequent second-generation biosensors were biomolecule based and used artificial redox mediators, such as organic dyes to detect and to increase the reproducibility and sensitivity of the result. However, the recent generation of biosensors work mostly on the principle of electron mobility, with different criteria, such as selectivity, precision, sensitivity, etc., can be used to quantify, efficiently. This review deals with exploring the scope and applications of Immobilized lipase biosensors. Generally, Triglycerides or TG molecules are either detected using Gas Chromatography or, using a chemical or an enzymatic assay. Immobilization of lipase on solid supports has led to increased stability and reusability of the enzyme in non-aqueous solvents. With better enzyme performance, efficient product recovery, and separation from the reaction, immobilized lipase biosensors are garnering increasing interest worldwide. Along with so many advantages including but not limiting to ones mentioned earlier, immobilized lipase-based biosensors come with their own set of challenges, such as the partitioning of the analyte with aqueous medium, slower reaction rate, etc., they have been discussed in the following review. Alongside, we also review the development of a new generation of biosensors and bioelectronic devices based on nanotechnology.