V. Lemesh, G. Mozgova, Yadviga E. Pilyuk, N. E. Horuzhiy, E. S. Byk, A. Burakova
{"title":"RLM和avrlm基因在油菜黑腿特异性抗性实现中的作用","authors":"V. Lemesh, G. Mozgova, Yadviga E. Pilyuk, N. E. Horuzhiy, E. S. Byk, A. Burakova","doi":"10.29235/1561-8323-2018-62-2-203-209","DOIUrl":null,"url":null,"abstract":"As part of the study, DNA markers for local races L. maculans were identified and found that the AvrLm4-7 sequence in the selected fungus population is specifically recognized by the resistance Rlm4 and Rlm7 genes. The SCAR marker BN204 was identified, which allows us to identify homozygous and heterozygous plants carrying the Rlm4 gene. The work collection, including 22 varieties and 39 hybrids of rape, was analyzed using the DNA marker BN204. PCR results allowed us to establish that the number of individual plants with the resistance Rlm4 gene was higher than the number of stable forms characterized by the infection of leaf explants with pathogen races carrying the AvrLm4-7 sequence. It is supposed that these individual plants contain the Rlm4 gene, but they lack the Rlm7 gene.","PeriodicalId":11227,"journal":{"name":"Doklady Akademii nauk","volume":"1 1","pages":"203-209"},"PeriodicalIF":0.0000,"publicationDate":"2018-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"THE ROLE OF RLM AND AVRLM GENES IN THE REALIZATION OF RAPE SPECIFIC RESISTANCE TO BLACKLEG\",\"authors\":\"V. Lemesh, G. Mozgova, Yadviga E. Pilyuk, N. E. Horuzhiy, E. S. Byk, A. Burakova\",\"doi\":\"10.29235/1561-8323-2018-62-2-203-209\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"As part of the study, DNA markers for local races L. maculans were identified and found that the AvrLm4-7 sequence in the selected fungus population is specifically recognized by the resistance Rlm4 and Rlm7 genes. The SCAR marker BN204 was identified, which allows us to identify homozygous and heterozygous plants carrying the Rlm4 gene. The work collection, including 22 varieties and 39 hybrids of rape, was analyzed using the DNA marker BN204. PCR results allowed us to establish that the number of individual plants with the resistance Rlm4 gene was higher than the number of stable forms characterized by the infection of leaf explants with pathogen races carrying the AvrLm4-7 sequence. It is supposed that these individual plants contain the Rlm4 gene, but they lack the Rlm7 gene.\",\"PeriodicalId\":11227,\"journal\":{\"name\":\"Doklady Akademii nauk\",\"volume\":\"1 1\",\"pages\":\"203-209\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-05-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Doklady Akademii nauk\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.29235/1561-8323-2018-62-2-203-209\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Doklady Akademii nauk","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.29235/1561-8323-2018-62-2-203-209","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
THE ROLE OF RLM AND AVRLM GENES IN THE REALIZATION OF RAPE SPECIFIC RESISTANCE TO BLACKLEG
As part of the study, DNA markers for local races L. maculans were identified and found that the AvrLm4-7 sequence in the selected fungus population is specifically recognized by the resistance Rlm4 and Rlm7 genes. The SCAR marker BN204 was identified, which allows us to identify homozygous and heterozygous plants carrying the Rlm4 gene. The work collection, including 22 varieties and 39 hybrids of rape, was analyzed using the DNA marker BN204. PCR results allowed us to establish that the number of individual plants with the resistance Rlm4 gene was higher than the number of stable forms characterized by the infection of leaf explants with pathogen races carrying the AvrLm4-7 sequence. It is supposed that these individual plants contain the Rlm4 gene, but they lack the Rlm7 gene.
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