Green Synthesis of Copper (Cu) Nanoparticles Using Marine Brown Algae Turbinaria Ornata and Its Brine Shrimp Lethality Bioassay

Objective: The synthesis of copper nanoparticles using marine macro algae has been unexplored, which resulted to nano sizes having the greatest potential for biomedical applications. In this investigation, we present the synthesis and brine shrimp lethality bioassay of Copper nanoparticles using Turbinaria ornate, marine algae. Methods: Fresh T. ornata was collected from the Mandapam, Southeast coast of India. The seaweed extract was sued for the synthesis of CuNPs at room temperature. The detail characterization of the nanoparticles was carried out, using Uvvis spectroscopy at 420nm. SEM analysis confirmed the range of particle size between 100-200nm. Fourier Transform Infra-Red (FTIR) spectroscopy analysis showed that the synthesized Copper nanoparticles are capped with biomolecule compounds which are responsible for reduction Copper ions. Results: The synthesized CuNPs were tested in vivo for their cytotoxic effect against the brine shrimp lethality bioassay and related toxicity result with them know cytotoxic activities. In the concentration of 0.75 mg/ml of Copper Nanoparticles of T. ornata about 50% shrimp remain survived after 24 hours. So LC50 value was seems to be 0.75 mg/ml. Conclusions: The above eco-friendly synthesis procedure of CuNPs could be easily scaled up in future for industrial and therapeutic needs.