枣椰树的抗氧化物稳定性(凤梨属)在加热时使用ABTS方法

Wieke Sriwulan
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引用次数: 0

摘要

自由基是一种对身体非常危险的化合物,因为它们会攻击并导致体内细胞损伤,因此需要抗氧化化合物来抵消这些自由基的存在。含有抗氧化化合物的水果之一是枣(Phoenix dactylifera L.),它含有类黄酮化合物,作为抗氧化剂。本研究的目的是测定其在400C、500C和650C加热温度下的抗氧化活性。本研究采用的提取方法为80%乙醇为溶剂的浸渍法。使用减少自由基的方法,即ABTS方法测试抗氧化活性,并使用维生素C作为对照来显示抗氧化活性。ABTS是测定过硫酸钾用二铵盐氧化得到的抗氧化活性的方法。采用波长为520 nm的紫外-可见分光光度计进行吸光度测定。采用线性回归方程进行数据分析,确定大枣(Phoenix dactylifera L.)中IC50值。IC50值表示样品中的抗氧化活性,即IC50值越小,样品中的抗氧化活性越高。采用Kruskal Wallis检验进行数据分析,其显著性值为0.104,即> 0.05,即各治疗组间无显著性差异。从分析数据的结果可知,加热温度对枣的抗氧化稳定性没有影响,线性回归方程中的抗氧化活性值也加强了这一点,其中各处理组的抗氧化活性值具有同样强的抗氧化活性值。基于Blois分类。关键词:凤尾草;抗氧化剂;提取物
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Stabilitas Antioksidan Buah Kurma (Phoenix dactylifera L.) Pada Suhu Pemanasan Dengan Metobe ABTS
Free radicals are compounds that are very dangerous for the body because they can attack and cause cell damage in the body, therefore antioxidant compounds are needed that can counteract the presence of these free radicals. One of the fruits that contain antioxidant compounds is dates (Phoenix dactylifera L.) which contain flavonoid compounds that act as antioxidants. The purpose of this study was to determine the antioxidant activity at heating temperatures of 400C, 500C, and 650C. The extraction method used in this study is the maceration method with 80% ethanol as solvent. Testing of antioxidant activity using the method of reducing free radicals, namely the ABTS method and using vitamin C as a comparison control to show antioxidant activity. ABTS is a method of determining antioxidant activity obtained from the oxidation of potassium persulfate with diammonium salt. Absorbance measurements were carried out using a UV-Vis spectrophotometer with a wavelength of 520 nm. Data analysis was carried out using linear regression equations to determine the IC50 value in the sample of dates (Phoenix dactylifera L.). The IC50 value indicates the antioxidant activity in a sample, namely the smaller the IC50 value, the higher the antioxidant in a sample. The results of data analysis using the Kruskal Wallis test obtained a significance value of 0.104 which indicates that > 0.05 which means there is no significant difference between each treatment group. From the results of the analysis data, it is known that there is no effect of heating temperature on the antioxidant stability of dates, this is also strengthened by the value of antioxidant activity in the linear regression equation where the value of antioxidant activity in each treatment group has an equally strong antioxidant activity value. based on the Blois classification. Keywords : Phoenix dactylifera L, Antioxidant and Extract 
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