The Effects of Trenbolone Supplementation on The Extremity Bones in Running Rats

ABSTRACT Anabolic steroids are testosterone derivatives through which anabolic effects are maintained and androgenic effects are minimized. The use of ergogenic agents is increasing among athletes for doping in order to increase physical performance and change external image. The objective of this study was to determine effects of trenbolone supplement administered on running rats for 4 weeks on extremity bones. The study was conducted with 28 male Wistar rats aged 28 days with a mean weight of 61,80 g supplied from the Selcuk University Experimental Medical Research and Application Center. The rate were divided into 4 groups as C (Controls), E (Exercise), T (Trenbolone), and TE (trenbolone + Exercise). The trial period lasted 4 weeks. Supply, care, feeding, and experimental applications of rats were performed in the Selcuk University Experimental Medical Research and Application Center. Anterior and posterior extremities’ bones were dissected and exposed, and the humerus and femur bones exposed were dried. Length, corpus thickness, cortex thickness, and medulla diameter points were determined and the necessary measures were taken. The results are expressed as mean ± SD. ANOVA and Duncan tests were used for the comparison of data. p 0.05). Results of this study indicate that trenbolone supplement may lead to early epiphyseal closure in femur and humerus bones of rats, ceasing the increase in their length. We believe that the results obtained from this trenbolone trial will provide important data to the studies that will be conducted on anabolic androgenic steroids.   Key words: Anabolic Androgenic Steroid, Trenbolone, Rat, Femur, Humerus