鱼腥草的纯化。精油大孔树脂微乳液包封提高其安全性和抗病毒活性

Jianmei Pang, Wu-jun Dong, Yuhuan Li, Xuejun Xia, Zhihua Liu, Huazhen Hao, Lingmin Jiang, Yuling Liu
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引用次数: 19

摘要

鱼腥草精油。由于其优良的生物活性,在中药中被广泛应用。然而,精油的杂质和缺陷制剂限制了其安全性和有效性。为此,我们提出了一种将溶剂萃取与大孔树脂灵活纯化相结合,然后用微乳液包封的策略,以安全有效地制备山茱萸精油(HEO)。通过正交试验和吸附-解吸试验对提取工艺和纯化工艺进行了优化。经验证,纯HEO中鱼腥草素的平均含量为44.3%±2.01%,具有较大的工业应用潜力。随后,采用高压均质法制备了纯heo负载微乳液,并对其进行了表征。结果表明,成功制备了负载heo的纯微乳液,平均粒径为179.1 nm,包封率为94.7%。此外,安全性评价试验和体外抗病毒试验表明,经D101树脂纯化后HEO的安全性和活性显著提高,并经微乳包封进一步提高。这些结果表明,采用大孔树脂纯化HEO并进行微乳液包封将是HEO用于抗病毒治疗的一种很有前景的工业应用方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Purification of Houttuynia cordata Thunb. Essential Oil Using Macroporous Resin Followed by Microemulsion Encapsulation to Improve Its Safety and Antiviral Activity
Essential oil extracted from Houttuynia cordata Thunb. (H. cordata) is widely used in traditional Chinese medicine due to its excellent biological activities. However, impurities and deficient preparations of the essential oil limit its safety and effectiveness. Herein, we proposed a strategy to prepare H. cordata essential oil (HEO) safely and effectively by combining the solvent extraction and the macroporous resin purification flexibly, and then encapsulating it using microemulsion. The extraction and purification process were optimized by orthogonal experimental design and adsorption-desorption tests, respectively. The average houttuynin content in pure HEO was then validated at 44.3% ± 2.01%, which presented a great potential for industrial application. Subsequently, pure HEO-loaded microemulsion was prepared by high-pressure homogenization and was then fully characterized. Results showed that the pure HEO-loaded microemulsion was successfully prepared with an average particle size of 179.1 nm and a high encapsulation rate of 94.7%. Furthermore, safety evaluation tests and in vitro antiviral testing indicated that the safety and activity of HEO were significantly improved after purification using D101 resin and were further improved by microemulsion encapsulation. These results demonstrated that the purification of HEO by macroporous resin followed by microemulsion encapsulation would be a promising approach for industrial application of HEO for the antiviral therapies.
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