GPCR氨基酸序列的理化性质为了解GPCR- g -蛋白偶联提供依据

IF 0.4 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Ganga D. Ghimire, Hideki Tanizawa, M. Sonoyama, S. Mitaku
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引用次数: 1

摘要

g蛋白偶联受体(gpcr)在配体激活后与g蛋白结合。了解gpcr与g蛋白特异性结合的机制是生物信息学研究的重要课题之一。本研究通过分析各区域的物理性质,对gpcr进行g蛋白家族分类,更好地了解其结合特异性。我们重点研究了细胞质环(IL1、IL2和IL3的N/ c端)、细胞外环(NTL、EL1和EL2的N/ c端)和跨膜螺旋的细胞质末端,但连接到c端环的螺旋除外。疏水性、电荷密度、赖氨酸和精氨酸密度以及环长度的分布使得gpcr的识别准确率超过90%。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Physicochemical properties of GPCR amino acid sequences for understanding GPCR-G-protein coupling
G-protein coupled receptors (GPCRs) bind with G-proteins upon activation by ligands. Understanding the mechanisms of specific binding between GPCRs and G-proteins is one of the most important issues in bioinformatics research. In this study, the physical properties of various regions were analyzed in order to classify GPCRs by G-protein family and to better understand binding specificity. We focused on cytoplasmic loops (IL1, IL2 and N/C-terminus of IL3), extracellular loops (NTL, EL1 and N/C-terminus of EL2) and cytoplasmic termini of transmembrane helices, except for helices that connect to C-terminus loops. The distribution of hydrophobicity, charge density, lysine and arginine densities, and loop length enabled discrimination of GPCRs with more than 90% accuracy.
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来源期刊
Chem-Bio Informatics Journal
Chem-Bio Informatics Journal BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
0.60
自引率
0.00%
发文量
8
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