一个多重系统的发展,以确定肥胖倾向的11个遗传标记

Q4 Chemistry
G. Sergeev, I. Haidukevich, A. Gorkavaya, M. Ameliyanovich, I. Mosse, S. Usanov
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引用次数: 0

摘要

建立了一个系统,通过微序列(SNaPshot分析)鉴定与肥胖风险相关的11个遗传标记:rs10852521、rs11075990、rs1121980、rs1421085、rs1477196、rs17817449、rs3751812、rs7206790、rs8047395、rs9940128 (FTO基因)和rs1137101 (LEPR基因)。优化扩增和微测序反应的条件及反应混合物的组成,使11个标记同时进行分析。所得到的系统经过测试,显示出检测这些多态性所需的高度可重复性和灵敏度。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Development of a multiplex system for determining 11 genetic markers of predisposition to obesity
A system has been developed to identify 11 genetic markers associated with the risk of obesity: rs10852521, rs11075990, rs1121980, rs1421085, rs1477196, rs17817449, rs3751812, rs7206790, rs8047395, rs9940128 (FTO gene) and rs1137101 (LEPR gene) by minisequencing (SNaPshot analysis). The conditions for carrying out the amplification and minisequencing reactions, as well as the compositions of the reaction mixtures, were optimized so that the analysis was carried out for all 11 markers simultaneously. The resulting system was tested and showed a high degree of reproducibility and sensitivity required for the detection of these polymorphisms.
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来源期刊
CiteScore
0.30
自引率
0.00%
发文量
38
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