HT074对HCl/EtOH所致胃损伤的抗溃疡作用

Young Sik Kim, H. J. Park, Jungbin Song, Donghun Lee, Hocheol Kim
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引用次数: 4

摘要

目的:研究菊白芍复方中药提取物(HT074)对酸化乙醇胃损伤的抗溃疡作用及其可能机制。方法:采用DPPH(2,2-二苯基-1-吡啶肼基)和ABTS(2,2'-氮基-双(3-乙基苯并噻唑-6-磺酸)自由基清除能力测定HT074及其成分的抗氧化活性。HT074按100、300 mg/kg剂量口服给药,每天2次,连续14 d后,采用酸化乙醇灌胃诱导大鼠胃病变。测定胃粘膜组织中超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)和谷胱甘肽(GSH)浓度等氧化应激标志物。同时检测胃粘膜组织中人黏液蛋白基因mucin 5AC (MUC5AC) mRNA的表达。结果:HT074对DPPH和ABTS自由基具有剂量依赖性的清除活性。HT074 300 mg/kg连续灌胃14 d,可显著降低HCl/EtOH致大鼠胃损伤及组织损伤。HT074处理显著提高了SOD活性(300 mg/kg)和GSH浓度(100和300 mg/kg),过氧化氢酶浓度无显著升高。HT074 100、300 mg/kg处理显著提高MUC5AC mRNA表达量。结论:HT074通过提高SOD活性、GSH浓度和粘蛋白生物合成水平,对酸化乙醇引起的胃黏膜氧化应激具有保护作用。这些结果提示HT074可能是预防和治疗胃炎和胃溃疡的有效候选药物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Anti-ulcer effects of HT074 on HCl/EtOH induced gastric injury
Objectives : This study aimed to investigate the anti-ulcer effect of an standardized herbal extracts mixture of Inulae Flos and Paeoniae Radix (HT074) on acidified ethanol induced gastric injury and its potential mechanisms. Methods : Antioxidant activities of HT074 and its constituents were measured by DPPH (2,2-Diphenyl-1-picrylhydrazyl) and ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging capacity. After the oral administration of HT074 at doses of 100, 300 mg/kg twice per day for 14 days, Gastric lesions were induced by oral administration of acidified ethanol in Sprague Dawley rats. Oxidative stress markers, such as super oxide dismutase (SOD) activity, concentrations of catalase (CAT) and glutathione (GSH) were measured in gastric mucosal tissues. Additionally, the expression of human mucin gene, Mucin 5AC (MUC5AC) mRNA in gastric mucosal tissues was measured. Results : HT074 showed dose dependent radical scavenging activities against DPPH and ABTS radicals. Oral administration of HT074 300 mg/kgfor 14 consecutive days significantly decreased gastric lesions and histological damages induced by HCl/EtOH in rats. HT074 treatment significantly increased the activity of SOD (300 mg/kg) and concentration of GSH (100 and 300 mg/kg), however catalase concentration was not significantly increased. MUC5AC mRNA expression was significantly increased by HT074 100, 300 mg/kg treatment. Conclusions : HT074 protects the gastric mucosa from oxidative stress caused by acidified ethanol by increasing the activity of SOD, concentration of GSH and mucin biosynthesis. These findings suggest that HT074 could be an effective candidate for prevention and treatment of gastritis and gastric ulcer.
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