百香果甲醇提取物的协同细胞毒作用。例b)和顺铂抗Hela细胞系

A. N. Artanti, U. H. Pujiastuti, F. Prihapsara, R. Rakhmawati
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引用次数: 5

摘要

宫颈癌是世界上主要的死亡原因之一,需要有效的治疗来降低其死亡率。在治疗中经常使用的化疗药物之一是顺铂。然而,由于耐药及其副作用,需要一种可以与顺铂联合的药物。Parijoto fruit (Medinilla speciosa Reinw.ex.Bl)含有次级代谢物化合物,具有潜在的抗癌作用。本研究旨在通过MTT法测定由IC50值计算的百香果甲醇提取物的细胞毒作用,并通过联合指数(CI)值评价与顺铂联合治疗的增效作用及其细胞活力。结果表明,Parijoto fruit甲醇提取物(MEP)对HeLa细胞具有细胞毒作用,IC50值为209.6 μg/mL,而顺铂对HeLa细胞的IC50值为12.8 μg/mL。MEP 26.205 ppm (1/8 IC50)与顺铂1.601 ppm (1/8 IC50)联合使用对HeLa细胞株具有协同作用,CI值为0.69。综上所述,基于MEP与顺铂的协同细胞毒性作用,MEP具有作为联合化疗药物的潜力。关键词:细胞毒性,麦地那,顺铂,联合化疗,MTT
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Synergistic Cytotoxicity Effect by Combination of Methanol Extract of Parijoto Fruit (Medinilla speciosa Reinw. ex. Bl) and Cisplatin Against Hela Cell Line
As one of the leading causes of death in worldwide, cervical cancer requires the effective therapies to reduce its mortality rate. One of the chemotherapy agents that frequently used in the treatment is cisplatin. However, due to drug resistance and its side effects, an agent that can be combined with cisplatin is needed. Parijoto fruit (Medinilla speciosa Reinw.ex.Bl) contains secondary metabolites compounds that have potential as anticancer. The study aims to determine the cytotoxic effect of methanol extract of Parijoto fruit calculated from the IC50 value and the synergicity of the combinational treatment with cisplatin evaluated from the Combination Index (CI) value and its cell viability by using MTT assay. Results showed that methanol extract of Parijoto fruit (MEP) performed cytotoxic effect on HeLa cell line with IC50 of 209.6 μg/mL while the value of IC50 of cisplatin against HeLa cells amounted to 12.8 μg/mL. The combination of 26.205 ppm (1/8 IC50) of MEP and 1.601 ppm (1/8 IC50) of Cisplatin performed synergistic effect on HeLa cell line with the CI value of 0.69. From the above results, it can be concluded that MEP is potential as co-chemotherapy agent based on the synergistic cytotoxicity effect with cisplatin.Keyword: cytotoxic, Medinilla speciosa, cisplatin, co-chemotherapy, MTT
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