一种特殊凉茶对雄激素绝育大鼠肥胖和无排卵的影响。

F. Sun, J. Yu
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引用次数: 23

摘要

一种特殊的凉茶已被用于治疗克罗米芬抗性无排卵疾病和肥胖,特别是对多囊卵巢综合征(PCOS)伴高胰岛素血症的病例有效。研究了凉茶对雄激素绝育大鼠(ASR)肥胖和无排卵的影响。9日龄Sprague-Dawley雌性大鼠皮下注射1.25 mg丙酸睾酮,建立ASR模型。大鼠在112日龄左右被处死。ASR表现为PCO、无排卵、高食物摄入、体重升高和肥胖。免疫细胞化学表明雌激素受体(ER)主要分布在视前区(POA)含神经肽Y (NPY)神经元的细胞质中,在弓状核(ARC)合成神经肽Y的神经元的细胞核和纤维中也发现了雌激素受体(ER)的共表达。与正常对照组相比,ASR大鼠NPY表达升高,下丘脑弧中隆起(ME) ER数量减少,ME促性腺激素释放激素(GnRH)水平降低,血清雌激素(E2)和瘦素(leptin)升高,卵泡刺激素(FSH)和黄体生成素(LH)水平显著降低。NPY与ER或GnRH、瘦素与FSH或LH呈显著负相关。血清瘦素与体重呈正相关。饲喂凉茶后,ASR的代谢-内分泌变化趋于正常化。ASR中肥胖和促性腺激素均有表达。卵巢异常激素环境(E2水平升高)可能导致NPY表达增强,GnRH和促性腺激素分泌减少。凉茶可减轻ASR患者体重,促进排卵。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The effect of a special herbal tea on obesity and anovulation in androgen-sterilized rats.
A special herbal tea has been used to treat clomiphene-resistant anovulatory disease and obesity effectively, especially in polycystic ovary syndrome (PCOS) cases with hyperinsulinemia. The effect of the herbal tea on obesity and anovulation was investigated in androgen-sterilized rats (ASR). The ASR model was established by subcutaneous injection of 1.25 mg testosterone propionate to Sprague-Dawley female rats at the age of 9 days. Rats were sacrificed around 112 days of age. ASR manifested with PCO, anovulation, high food intake, elevated body weight, and obesity. Immunocytochemistry demonstrated that estrogen receptors (ER) were predominantly distributed in the cytoplasm of neuropeptide Y (NPY)-containing neurons in the preoptic area (POA), and the coexpression was also found in the nuclei and fibers of NPY-synthesizing neurons in the arcuate nucleus (ARC). Compared with that in normal control rats, NPY expression was increased, the numbers of ER in hypothalamic ARC-median eminence (ME) decreased, gonadotropin-releasing hormone (GnRH) levels in ME was decreased, serum estrogen (E2) and leptin were elevated, and follicular stimulating hormone (FSH) and luteinizing hormone (LH) levels were reduced significantly in ASR. Significantly negative correlations between NPY and ER or GnRH, and between leptin and FSH or LH were observed. A positive correlation existed between serum leptin and body weight. These metabolic-endocrine changes in ASR were normalized after feeding the herbal tea. Both obesity and hypogonadotropin were expressed in ASR. The abnormal ovarian hormone milieu (elevated E2 levels) may have enhanced NPY expression and resulted in less GnRH and gonadotropin secretion. The herbal tea reduced body weight and induced ovulation in ASR.
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