{"title":"转鼓式生物反应器固态发酵黑曲霉生产豆粕植酸酶的研究","authors":"Sukanya Saithi , Anan Tongta","doi":"10.1016/j.aaspro.2016.12.005","DOIUrl":null,"url":null,"abstract":"<div><p>Soybean meal is an agro-industrial residue which is used as a substrate to produce the enzyme phytase by <em>Aspergillusniger</em> in solid-state fermentation. Phytase is a biocatalytic enzyme widely used in the animal feed industry. The aim of this study was to investigate the growth of <em>A. niger</em> and its production of phytase in two systems: 500<!--> <!-->mL Erlenmeyer flasks (EF) and a 600 L rotating drum bioreactor (RDB). The highest phytase activity found was about 1,200 unit/g substrate dry weight in EF and 580 unit/g substrate dry weight in RDB. In solid-state fermentation it is necessary to control temperature and substrate moisture content to optimize <em>A. niger</em> growth and its production of phytase. This study confirmed that temperature and moisture content of substrate could be controlled to optimize the result during fermentation in a 600 L RDB. In this study, inlet air velocity was controlled at 1.5 m/s and the rotational speed at 1.0<!--> <!-->rpm in solid-state fermentation. The crude enzyme produced by <em>A. niger</em> in solid-state fermentation was achieved in a 600 RDB after 36<!--> <!-->h of fermentation. This crude enzyme was dried in a hot air oven at 50<sup>o</sup>C for 3 d prior to use in animal feed. Phytase activity was found to be 1.5-fold lower than the activity obtained before drying the crude enzyme.</p></div>","PeriodicalId":100063,"journal":{"name":"Agriculture and Agricultural Science Procedia","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.aaspro.2016.12.005","citationCount":"19","resultStr":"{\"title\":\"Phytase Production of Aspergillus Niger on Soybean Meal by Solid-State Fermentation Using a Rotating Drum Bioreactor\",\"authors\":\"Sukanya Saithi , Anan Tongta\",\"doi\":\"10.1016/j.aaspro.2016.12.005\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Soybean meal is an agro-industrial residue which is used as a substrate to produce the enzyme phytase by <em>Aspergillusniger</em> in solid-state fermentation. Phytase is a biocatalytic enzyme widely used in the animal feed industry. The aim of this study was to investigate the growth of <em>A. niger</em> and its production of phytase in two systems: 500<!--> <!-->mL Erlenmeyer flasks (EF) and a 600 L rotating drum bioreactor (RDB). The highest phytase activity found was about 1,200 unit/g substrate dry weight in EF and 580 unit/g substrate dry weight in RDB. In solid-state fermentation it is necessary to control temperature and substrate moisture content to optimize <em>A. niger</em> growth and its production of phytase. This study confirmed that temperature and moisture content of substrate could be controlled to optimize the result during fermentation in a 600 L RDB. In this study, inlet air velocity was controlled at 1.5 m/s and the rotational speed at 1.0<!--> <!-->rpm in solid-state fermentation. The crude enzyme produced by <em>A. niger</em> in solid-state fermentation was achieved in a 600 RDB after 36<!--> <!-->h of fermentation. This crude enzyme was dried in a hot air oven at 50<sup>o</sup>C for 3 d prior to use in animal feed. Phytase activity was found to be 1.5-fold lower than the activity obtained before drying the crude enzyme.</p></div>\",\"PeriodicalId\":100063,\"journal\":{\"name\":\"Agriculture and Agricultural Science Procedia\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2016-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.aaspro.2016.12.005\",\"citationCount\":\"19\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Agriculture and Agricultural Science Procedia\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2210784316302893\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Agriculture and Agricultural Science Procedia","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2210784316302893","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 19
摘要
豆粕是一种工农业废渣,被黑曲霉用作固体发酵生产植酸酶的底物。植酸酶是一种广泛应用于动物饲料工业的生物催化酶。本研究的目的是研究黑曲霉在500ml Erlenmeyer烧瓶(EF)和600l旋转鼓式生物反应器(RDB)两种系统中的生长和植酸酶的生产。植酸酶活性最高的品种为EF和RDB,分别为1200单位/g和580单位/g。在固态发酵中,需要控制温度和底物含水量,以优化黑曲霉的生长和植酸酶的产量。本研究证实,在600 L RDB发酵过程中,可以通过控制底物的温度和含水量来优化发酵效果。在本研究中,固体发酵的进风速度控制在1.5 m/s,转速控制在1.0 rpm。黑曲霉固态发酵36 h后,在600 RDB条件下获得粗酶。在用于动物饲料之前,将该粗酶在50℃的热风烘箱中干燥3天。植酸酶活性比粗酶干燥前的活性低1.5倍。
Phytase Production of Aspergillus Niger on Soybean Meal by Solid-State Fermentation Using a Rotating Drum Bioreactor
Soybean meal is an agro-industrial residue which is used as a substrate to produce the enzyme phytase by Aspergillusniger in solid-state fermentation. Phytase is a biocatalytic enzyme widely used in the animal feed industry. The aim of this study was to investigate the growth of A. niger and its production of phytase in two systems: 500 mL Erlenmeyer flasks (EF) and a 600 L rotating drum bioreactor (RDB). The highest phytase activity found was about 1,200 unit/g substrate dry weight in EF and 580 unit/g substrate dry weight in RDB. In solid-state fermentation it is necessary to control temperature and substrate moisture content to optimize A. niger growth and its production of phytase. This study confirmed that temperature and moisture content of substrate could be controlled to optimize the result during fermentation in a 600 L RDB. In this study, inlet air velocity was controlled at 1.5 m/s and the rotational speed at 1.0 rpm in solid-state fermentation. The crude enzyme produced by A. niger in solid-state fermentation was achieved in a 600 RDB after 36 h of fermentation. This crude enzyme was dried in a hot air oven at 50oC for 3 d prior to use in animal feed. Phytase activity was found to be 1.5-fold lower than the activity obtained before drying the crude enzyme.