拟Acroporid细菌共生体对褐带病相关细菌的抑菌活性

Q3 Environmental Science
R. Amalia, D. Ayuningrum, A. Sabdono, O. Radjasa
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引用次数: 1

摘要

由于珊瑚疾病,如褐带病(BrBD),印度尼西亚大部分地区的珊瑚礁状况一直在下降。BrBD的治疗包括使用对致病因子具有拮抗特性的生物防治剂。本研究旨在从健康硬珊瑚中分离出与BrBD相关的细菌,以及具有抗BrBD生物活性的细菌。2015年3月,在Karimunjawa海洋国家公园对珊瑚和BrBD进行了取样和鉴定。从健康珊瑚和感染珊瑚中分离和纯化细菌。采用覆盖法和琼脂扩散法对分离株进行抗致病性测定。最后利用16S rRNA基因扩增对活性菌进行分子鉴定。从健康珊瑚中分离出多达57株细菌,从有BrBD症状的珊瑚中分离出4株细菌。共分离出15株(26%)具有抗brbd相关细菌的活性。通过16S rRNA基因序列鉴定出抗病原菌活性最强的3株菌株GAMSH 3、KASH 6和TAPSH 1。结果表明,它们分别与marismortui(97%)、iheyensis(97%)和蜡样芽孢杆菌(96%)一致。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antipathogenic Activity of Acroporid Bacterial Symbionts Against Brown Band Disease-Associated Bacteria
The coral reefs’ condition in most regions in Indonesia has been declining due to coral diseases, such as Brown Band Disease (BrBD). A treatment for BrBD involves the use of biological control agents that have antagonistic properties against disease-causing agents. This study aimed to isolate bacteria from healthy hard coral, those associated with BrBD, and those that had bioactivities against BrBD. Sampling and identification of corals and BrBD were carried out in March 2015 at the Marine National Park of Karimunjawa. Bacteria from healthy and infected corals were isolated and purified. The isolates were subjected to antipathogenic assay using overlay and agar diffusion methods. Finally, molecular identification of active bacteria was carried out using the 16S rRNA gene amplification. As many as 57 bacterial isolates were obtained from healthy coral, as well as four bacterial isolates from coral with BrBD symptoms. A total of 15 bacterial isolates (26%) showed antipathogenic activity against BrBD-associated bacteria. Three isolates with the strongest antipathogenic activities, i.e., GAMSH 3, KASH 6, and TAPSH 1 were identified by 16S rRNA gene sequences. The results showed that they were aligned to Virgibacillus marismortui (97%), Oceanobacillus iheyensis (97%), and Bacillus cereus (96%), respectively.
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来源期刊
CiteScore
1.40
自引率
0.00%
发文量
10
审稿时长
16 weeks
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