槲皮素和熊去氧胆酸对大鼠肝缺血再灌注损伤的保护作用

Fares E. M. Ali, Amira M. Abo-Youssef, Basim As Messiha, R. Hemeda
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引用次数: 7

摘要

本研究旨在评价槲皮素和熊去氧胆酸(UDCA)与标准剂n -乙酰半胱氨酸(NAC)相比对大鼠肝缺血再灌注(IR)损伤的保护作用。简单地将大鼠分为5组,即假对照组、IR对照组、NAC组、槲皮素组和UDCA组。评估的生物标志物包括作为肝细胞完整性参数的血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、碱性磷酸酶(ALP)、乳酸脱氢酶(LDH)和总胆红素(tBil),作为炎症生物标志物的血清肿瘤坏死因子-α (TNF-α)、白细胞介素-6 (IL-6)、环氧化酶- ii (COX-II)和脂氧化酶(LOX),以及作为炎症生物标志物的肝髓过氧化物酶(MPO)和一氧化氮终产物(NOx),肝脏丙二醛(MDA)、谷胱甘肽还原(GSH)、过氧化氢酶(CAT),超氧化物歧化酶(SOD)和谷胱甘肽- s -转移酶(GST)作为氧化应激生物标志物,最后是肝三磷酸腺苷(ATP)作为能量储存生物标志物。为了证实生化估计的结果,进行了组织病理学研究。结果显示,槲皮素和UDCA可显著降低肝细胞损伤,显著降低血清ALT、AST、ALP、LDH、tBil、TNF-α、IL-6、COX-II和LOX水平,显著降低肝脏MPO、NOx和MDA水平,显著升高肝脏GSH、CAT、SOD、GST和ATP水平。槲皮素在大部分参数上的效果明显优于UDCA。组织病理学结果有力地支持了生化估计的结果。综上所述,槲皮素和UDCA对大鼠肝脏IR损伤具有抗氧化、抗炎和保能作用,且槲皮素效果较好,具有进一步的临床应用前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Protective Effects of Quercetin and Ursodeoxycholic Acid on HepaticIschemia-Reperfusion Injury in Rats
This study aims to evaluate the protective effects of Quercetin and Ursodeoxycholic acid (UDCA), as compared to standard agent N-acetylcysteine (NAC), on hepatic ischemia-reperfusion (IR)-induced injury in rats. Briefly, rats were divided into five groups, namely sham control, IR control, NAC, Quercetin and UDCA groups. Assessed biomarkers included serum alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and total bilirubin (tBil) as hepatocyte integrity parameters, serum tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), cyclooxygenase-II (COX-II) and Lipooxygenase (LOX), and hepatic myeloperoxidase (MPO) and nitric oxide end products (NOx) as inflammatory biomarkers, hepatic malondialdhyde (MDA), glutathione reduced (GSH), catalase (CAT), superoxide dismutase (SOD) and glutathione-S-transferase (GST) as oxidative stress biomarkers, and finally hepatic adenosine triphosphate (ATP) as energy store biomarker. To confirm results of biochemical estimations, a histopathological study was conducted. Results showed that Quercetin and UDCA significantly reduced hepatocyte injury evidenced by significant reductions in serum ALT, AST, ALP, LDH, tBil, TNF-α, IL-6, COX-II and LOX levels, significant reductions in hepatic MPO, NOx and MDA levels, and significant elevations in hepatic GSH, CAT, SOD, GST and ATP levels. Quercetin effect was significantly better than UDCA effect on most parameters. Histopathological findings strongly supported results of biochemical estimations. In conclusion, Quercetin and UDCA, with Quercetin being better, can protect against hepatic IR injury in rats, at least through anti-oxidant, anti-inflammatory and energypreserving effects, and may be promising for further clinical trials.
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