临床稳定无症状特应性哮喘患者CD4和CD8 T淋巴细胞上特定极迟抗原整合素亚基表达升高

S. Bazan-Socha, J. Żuk, J. Musial
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引用次数: 0

摘要

在哮喘中,炎症细胞从血液募集到气道是由粘附分子介导的,如选择素和整合素。细胞运输中最重要的整合素是含有α4和β2亚基的分子。我们推测α1β1和α2β1整合素(均在哮喘患者血嗜酸性粒细胞中发现)在哮喘发病中也起重要作用。目的:观察稳定型特应性哮喘患者外周血CD4和CD8 T淋巴细胞中选择性甚晚抗原(VLA)亚基α1、α2、α4和β1的表达。材料和方法:采用流式细胞术对25名成人特应性哮喘患者(临床状态稳定的轻中度持续性哮喘)和17名匹配的健康对照进行研究。结果:哮喘患者CD4 T细胞α4、β1表达明显高于对照组。血淋巴细胞中缺乏α1亚基。正常人淋巴细胞中几乎检测不到的α2链在哮喘患者中明显存在。令人惊讶的是,在患有哮喘超过4年的受试者(n = 15)中,在CD4和CD8 T细胞上均观察到α2、α4和β1的过表达。结论:选定的VLA亚基在血T细胞上的表达可能与哮喘持续时间有关。α2β1整合素在哮喘中的生物学作用尚不清楚,但由于α2β1整合素被描述为气道中胶原积累的刺激物,因此α2β1整合素可能至少部分地负责哮喘气道重塑。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Original paper Increased expression of selected very late antigen integrin subunits on CD4 and CD8 blood T lymphocytes in patients with clinically stable asymptomatic atopic asthma
Introduction: Recruitment of the inflammatory cells from blood to the airways in asthma is mediated by adhesive molecules, e.g. selectins and integrins. The most important integrins in cells trafficking are molecules containing α4 and β2 subunits. We hypothesized that also α1β1 and α2β1 integrins (both found by us on blood eosinophils of asthmatic subjects) are important in asthma pathogenesis. Aim: To assess the expression of selected very late antigen (VLA) subunits (α1, α2, α4 and β1) on blood CD4 and CD8 T lymphocytes from stable atopic asthmatic patients. Material and methods: The study was conducted on 25 adult atopic asthmatics (mild to moderate persistent asthma in a stable clinical condition) and 17 matched healthy controls using flow cytometry. Results: Expression of α4 and β1 on CD4 T cells was significantly higher in asthma than in controls. The α1 subunit was absent from blood lymphocytes. The α2 chain hardly detected on lymphocytes from healthy subjects was distinctively present in asthmatics. Surprisingly, in subjects suffering from asthma for longer than 4 years (n = 15), the overexpression of α2, α4 and β1 was observed on both: CD4 and CD8 T cells. Conclusions: Expression of selected VLA subunits on blood T cells may depend on asthma duration. The biological role of α2β1 integrin in asthma is unknown, but as it was described as a stimulator of collagen accumulation in the airways, α2β1 integrin could be, at least in part, responsible for asthma airway remodelling.
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