用于细胞外ATP高灵敏度体外成像的氧化还原型无标签ATP图像传感器

H. Doi, T. Horio, Y. Choi, Kazuhiro Takahashi, T. Noda, K. Sawada
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引用次数: 0

摘要

为了降低脑细胞外ATP动态成像的检测限制,我们开发了一种具有合理空间分辨率($37.3\ \ \mu\ mathm {m}$-pitch)的氧化还原型、高灵敏度、无标记ATP图像传感器,有效地用于体外实验。我们的传感器基于过氧化氢检测技术,使用氧化还原酶,最低可检测浓度为$3\ \mu \ mathm {M}$。此外,根据使用10 mM-HEPES缓冲液进行的体外生物实验结果,我们的传感器与现有的基于氢离子(H+)检测技术的成像设备相比,性能优越100倍。我们的新型ATP图像传感器有望成功应用于脑神经组织和细胞释放的ATP的高灵敏度成像。它在ATP的时空分析中也有潜在的应用前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Redox-Type Label-Free ATP Image Sensor for Highly Sensitive in Vitro Imaging of Extracellular ATP
To decrease the limit of detection of imaging of extracellular ATP dynamics in the brain, we developed a redox-type, highly sensitive, label-free ATP image sensor with a reasonable spatial resolution ($37.3\ \ \mu\mathrm{m}$-pitch) effective utilization in in-vitro experiments. The lowest detectable concentration achieved in our sensor, which is based on hydrogen peroxide detection techniques using redox enzymes, was $3\ \mu \mathrm{M}$. Further, according to the results of the in-vitro biological experiment conducted using a 10 mM-HEPES buffer, our sensor demonstrated a 100 times superior performance compared to the existing imaging devices that were also based on hydrogen ion (H+) detection techniques. It is expected that our novel ATP image sensor can be successfully applied for highly sensitive imaging of ATP released from brain nerve tissues and cells. It also has potential application in the spatiotemporal analysis of ATP.
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