尼日利亚Saki-East地方政府地区6至11岁儿童犬弓形虫血清阳性率及寄生虫对血浆细胞因子的影响

M. Olaniyan, Mufutau Azeez
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引用次数: 2

摘要

研究背景:犬弓形虫是一种被称为狗虫的蠕虫寄生虫,通过被感染的狗粪便或被污染的泥土感染人类。在经济条件较差的地区,这是一种被忽视的疾病。寄生虫感染可产生炎症过程。目的与目的:研究尼日利亚Saki-East地区6-11岁儿童犬形虫的血清阳性率及寄生虫对血浆细胞因子的影响。材料与方法:在Saki-East地方政府辖区招募了200名6-11岁的儿童(女100岁,男100岁)。其中161例未感染任何传染因子作为对照。取痰、血、粪便、皮肤切片和尿液样本进行显微镜鉴定。痰液采用Ziehl-Neelsen染色法检测抗酸杆菌,ELISA法检测犬t型肝炎病毒、抗丙型肝炎病毒(HCV)、乙肝表面抗原(HBsAg)、HIV1-p24抗原、肿瘤坏死因子α (TNFα)、白细胞介素-10。结果:调查结果显示,儿童犬弓形虫血清总阳性率为9.5%(19例),其中男童12例(6%),女童7例(3.5%)。其中5.5%(11)为单犬绦虫感染;1%(2)为犬弓形虫合并蚓样蛔虫感染;犬t型犬合并乙型肝炎病毒感染占1% (2);1%(2)为犬弓形虫与疟原虫共感染;犬弓形虫与钩虫共感染0.5%(1例);犬弓形虫与血血吸虫共感染0.5%(1例)。这些儿童没有同时感染艾滋病毒或丙型肝炎病毒。10%(20例)感染了感染性病原体,但犬弓形虫血清检测呈阴性。犬单绦虫感染患儿血浆细胞因子TNFα、IL-10较对照组显著升高(P < 0.05)。结论:犬t型绦虫总血清阳性率为9.5%,其中单感染犬t型绦虫5.5%,单感染犬t型绦虫血浆TNFα和IL-10显著升高。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Seroprevalence of Toxocara canis and the parasitic effect on plasma cytokines in children aged 6 to 11 years in Saki-East local government area in Nigeria
Study Background: Toxocara canis is a helminths parasite known as dog worm but infects human through infected dog feces or contaminated dirt. It is a neglected disease in economically less privileged areas. The parasitic infection can generate inflammatory process. Aim and Objective: This work was designed to determine the seroprevalence of T. canis and the parasitic effect on plasma cytokines in children aged 6–11 years in Saki-East local government area in Nigeria. Materials and Methods: Two hundred children aged 6–11 years (female – 100 and male – 100) were recruited across Saki-East local government area. One hundred and sixty-one of them not infected with any of the infectious agents were studied as control. Sputum, blood, stool, skin snip, and urine samples were obtained for microscopic identification of parasites. Sputum sample was also used for Ziehl–Neelsen staining to demonstrate acid-fast bacilli, while ELISA technique was used for determination of T. canis, anti-hepatitis C virus (HCV), HBsAg, HIV1-p24 antigen, tumor necrosis factor-alpha (TNFα), and interleukin (IL)-10. Results: The results obtained showed overall seroprevalence of T. canis among the children as 9.5% (19) including 6% (12) male and 3.5% (7) female children. This included 5.5% (11) monoinfected with T. canis; 1% (2) were T. canis coinfected with Ascaris lumbricoides; 1% (2) were T. canis coinfected with hepatitis B virus; 1% (2) were T. canis coinfected with Plasmodium spp.; 0.5% (1) were T. canis coinfected with hookworm; and 0.5% (1) were T. canis coinfected with Schistosoma haematobium. There is no coinfection with either HIV or HCV among the children. 10% (20) were infected with infectious agents but seronegative to T. canis. There was a significant increase in the plasma values of cytokines TNFα and IL-10 in T. canis monoinfected children compared with the control (P < 0.05). Conclusion: The work revealed an overall seroprevalence of T. canis as 9.5% including 5.5% monoinfection and a significant increase in plasma TNFα and IL-10 in T. canis monoinfection.
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