Antioxidant and Antihypertensive Activity of Collagen and Elastin Hydrolysate at Different Molecular Weights

The angiotensin-converting enzyme (ACE) inhibitory activity and antioxidant property of collagen and elastin hydrolysates, and their peptide fractions (< 5 kDa, 5-10 kDa, 10-100 kDa) were compared. The bovine raw-hide and paddywhack (Ligamentum nuchae) were used for the preparation of collagen and elastin hydrolysates, respectively. Unfractionated collagen and elastin hydrolysates (4 mg/mL) could reduce ACE activity by 61% and 58%. As a result of fractionation, ACE inhibitory activity of collagen hydrolysate was increased up to 85%, while this effect was not observed on elastin hydrolysate. Elastin hydrolysate showed intense radical scavenging abilities through 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-Azinobis-(3-Ethylbenzthiazolin-6-Sulfonic Acid (ABTS) assay. The collagen hydrolysate obtained from the bovine rawhide has more perspective for the bioactive food additive having ACEI activity in comparison with the elastin hydrolysate.