单峰骆驼不同卵泡群卵泡液生化成分及其与血药浓度的关系

M. Shehab-El-Deen, S. Al-Dobaib, K. Al-Sobayil
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引用次数: 2

摘要

卵泡液代谢谱的变化可作为卵母细胞和颗粒细胞质量的间接指标。本研究旨在探讨单峰骆驼不同生长阶段卵泡中卵泡液的生化成分及其与血清生化成分的关系。卵巢采集自当地屠宰场(Oniza, KSA)。屠宰后不久,采集单峰骆驼(n = 20)的血液样本,并从三组不同的非闭锁卵泡(直径4-6 mm、6-8mm和10-20mm)中抽取卵泡液。通过保持卵泡大小来收集卵泡样本。测定血清和卵泡液样品中葡萄糖、胆固醇、甘油三酯、尿素、总蛋白、乳酸脱氢酶(LDH)、皮质醇、三碘甲状腺原氨酸(T3)、胰岛素样生长因子-1 (IGF-1)和非酯化脂肪酸(NEFA)的浓度。无论卵泡大小,卵泡液中葡萄糖、胆固醇、甘油三酯、总蛋白、LDH、T3、IGF-1和NEFA浓度均下降,而尿素浓度与血清相比呈上升趋势。与大、中滤泡组相比,小滤泡组IGF-1浓度梯度显著。我们本研究的数据表明,单峰骆驼的卵母细胞和颗粒细胞在一个生化环境中发育,除了IGF-1外,从小卵泡到大卵泡没有明显的变化。综上所述,上述生长卵泡代谢变化与血液代谢变化有关,可用于测定单峰骆驼卵泡优势度、卵母细胞和颗粒细胞质量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Biochemical Compositions in the Follicular Fluid of Different Follicle Groups and Their Relationship with the Blood Concentrations in Dromedary Camels
Metabolic profile changes of the follicular fluid of the growing follicles can be used as an indirect indicator of the oocyte and granulosa cells quality. The aim of this study was to investigate the biochemical compositions of follicular fluid collected from follicles at different stages of growth and their relationship with that of blood serum in Dromedary camel. Ovaries were colleceted from local slaughterhouse (Oniza, KSA). Soon after slaughtering, blood samples were collected from Dromedary camel (n = 20) and follicular fluid was aspirated from three different groups of non-atretic follicles (4–6)mm, 6–8mm and 10–20mm diameter). Follicular samples were pooled by maintaining the follicular sizes. Concentrations of glucose, cholesterol, triglycerides, urea, total protein, lactate dehydrosenase (LDH), cortisol, triiodothyronine (T3), insulin-like growth factor-1 (IGF-1) and non-esterified fatty acids (NEFA) were assayed in each serum and follicular fluid sample. The concentrations of glucose, cholesterol, triglycerides, total protein, LDH, T3, IGF-1 and NEFA decreased in the follicular fluid irrespective of follicular sizes and increasing in trend for urea in comparison with blood serum. There was a significant concentration gradient for IGF-1 in small follicular group compared to large or medium groups. Our data from the present study suggest that the oocyte and the granulosa cells of Dromedary she camel develop in a biochemical environment that does not have remarkable changes from small to large follicles except for IGF-1. In conclusion, the above-mentioned metabolic changes in the growing follicle is related to blood metabolic changes and, therefore, may be used in determining follicular dominance, oocyte and granulosa cells quality in Dromedary camel.
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