利用色谱方法鉴定新化合物并筛选抗糖尿病和抗氧化活性

A. Patil, G. Janvale, D. Bhusari, S. Shinde
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引用次数: 1

摘要

本研究对水合田叶柱洗脱组分的植物化学筛选、柱层析、薄层色谱研究、蛋白酶活性、抗炎活性、抗糖尿病活性和抗氧化活性进行了评价。植物化学筛选反映了生物碱、类黄酮、香豆素、萜类、类固醇、大黄素、醌类的存在。采用柱层析法纯化生物活性化合物。采用正丁醇、乙酸、丙酮等不同极性溶剂体系进行了薄层色谱研究。TLC谱图在254nm和366nm处显示纯带。纯化和洗脱组分均表现出较强的“蛋白水解活性”。体外抗炎活性以白蛋白变性部分3评价,在500µg/ml浓度下,活性最高的部分为75%,其次为5(62.73%);膜稳定试验部分6(80.23%)其次为3(64.65%);蛋白酶抑制活性部分5(88%)其次为7(87.68%)。阿司匹林(90.87%)作为抗炎活性研究的标准药物。采用α淀粉酶抑制法测定其体外抗糖尿病活性。在浓度为500µg/ml时,活性最高的是组分4(79.05%)和组分5(77.05%)。通过还原功率测定2号组分的抗氧化活性,在500µg/ml时具有较高的吸光度1.04,然后将柱洗脱的还原功率与抗坏血酸作为标准进行比较,在500µg/ml时具有较高的吸光度0.93。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Bioprospecting of Adhatoda vasica for Identification of Novel Compounds using Chromatographic Methods and Screening for Anti-diabetic and Antioxidant Activity
This investigation column eluted fractions of leaf Adhatoda vasica of was assessed for its phytochemical screening, column chromatography, thin layer chromatographic studies, protease activity, anti-inflammatory activity, antidiabetic activity and antioxidant activity. Phytochemical screening reflects the presence of alkaloid, flavonoids, coumarins, terpenoids, steroids, emodin’s, Quinone’s. Column chromatography method was used for purification of bioactive compounds. Thin layer chromatographic study was carried out by using various solvent system of different type of polarity of n- butanol, acetic acid and acetone. TLC profiling shows pure band at 254nm and 366 nm. The strong “proteolytic activity” also pointed out in purified fraction of eluted fraction. In vitro anti-inflammatory activity was evaluated using albumin denaturation fraction 3, showing highest activity 75% followed by fraction 5 (62.73%), membrane stabilization assay fraction 6 (80.23%) followed by fraction 3 (64.65%) and proteinase inhibitory activity of fraction 5(88%) followed by fraction 7 (87.68%) at concentration 500 µg/ml. Aspirin (90.87%) was used as standard drug for the study of anti-inflammatory activity. In vitro antidiabetic activity was performed using Alfa amylase inhibition assay. Highest activity was showed in fraction 4 (79.05 %) and fraction 5 (77.05 %) at concentration 500 µg/ml. Antioxidant activity was performed by reducing power assay fraction number 2 has higher absorbance 1.04 at 500µg/ml followed by reducing power of column eluted fraction was compared with ascorbic acid as standard showing higher absorbance 0.93 at 500µg/ml.
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