全自动电泳装置(常光CTE8000)临床检测蛋白质性能评价

京子 宮﨑, 正芳 米山, 美穂 高橋, 照夫 江上, 宏明 大西, 卓 渡邊
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引用次数: 0

摘要

最近,东洋Roshi Kaisha有限公司开发了一种新的用于电泳分析蛋白质组分的醋酸纤维素膜,名为SELECA-VSP。在商业化供应之前,我们组织了一个研究小组,将这种新膜与日本广泛使用的蛋白质组分测定标准膜SEPARAX-SP进行比较。作为本课题组的一员,我们实验室在分析血清、腹水、胸水、脑脊液和尿液中的蛋白质组分时,主要研究了两种膜的差异。采用JOKO-CTE8000作为蛋白质组分分析仪。用缺乏EDTA的缓冲液对血清和腹水进行电泳时,β部分与其他部分的相关率较低。然而,当含edta缓冲液用于血清和腹水分析时,β-组分中两膜之间的相关性提高到与其他组分相当的水平。两种膜之间的相关性在除血清和腹水以外的样品分析中是令人满意的,无论缓冲液中是否存在EDTA。结果表明,该膜具有与标准膜相似的性能,可用于临床样品中蛋白质组分的测定。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
全自動電気泳動装置(常光CTE8000)による臨床検査タンパク性能評価
Recently a new cellulose acetate membrane for electrophoresis to analyze protein fractions, named SELECA-VSP, was developed by Toyo Roshi Kaisha Ltd. In advance of commercial supply, a study group was organized to investigate utility of this new membrane in comparison with SEPARAX-SP, a standard membrane for protein fraction assay widely used in Japan. As a member of this study group, our laboratory mainly investigated the difference between the two membranes when analyzing protein fractions in serum as well as ascites, pleural fluid, cerebrospinal fluid and urine. JOKO-CTE8000 was used as a protein fraction analyzer. When buffer lacking EDTA was used in electrophoresis of serum and ascites, the correlation efficiency between the two membranes in percentage of the β fraction was inferior to that of other fractions. When EDTA-containing buffer was used in analysis of serum and ascites, however, the correlation between the two membranes in β-fraction was improved to a comparable level to other fractions. The correlations between the two membranes were satisfactory in analysis of samples other than serum and ascites, regardless of the presence of EDTA in buffer. We conclude that this newly developed membrane has the similar performance to a standard membrane and could be applied in assay of protein fraction in clinical samples.
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