豚鼠硬脑膜和硬脑膜品种果实油脂化学成分及抗氧化性能的评价

U. L. Onyebuchi-Ogwuegbu, D. I. Ukairo, C. Ibegbulem
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摘要

棕榈油和棕榈仁油的药用价值一直受到尼日利亚东南部农村居民的好评。采用标准方法研究了不同品种油的植物化学成分和抗氧化性能。油样包括:DPO(硬脑膜棕榈油)、TPO(硬脑膜棕榈油)、NDPKO(硬脑膜原生棕榈仁油)、NTPKO(天然硬脑膜棕榈仁油)、HDPKO(硬脑膜棕榈仁油己烷提取物)和HTPKO(硬脑膜棕榈仁油己烷提取物)。结果显示,所有油样品中均含有甾体、苷类、萜类、三萜和酚类物质,而皂苷、黄酮类和香豆素仅在DPO、TPO和NDPKO中检测到。定量植物化学分析结果显示,NDPKO中萜类含量最高(1.77±0.14 mg/kg),三萜含量最高(288.50±23.30 mg/100g)。DPO中皂苷、黄酮类和香豆素含量最高,分别为1.00±0.001 mg/kg、308.30±1.81 mg/100g和43.98±0.13µg/100g。其中,甾体成分含量最高的是NTPKO(150.40±0.24 mg/100g),苷类成分含量最高的是TPO(9.01±0.07 mg/100g),酚类成分含量最高的是45.04±0.12 mg/100g (p<0.05)。TPO中β-胡萝卜素(2.06±0.00 mg/100ml)和维生素A(105.60±0.15 IU/100ml)含量最高,维生素E(3.73±0.30µg/ml)含量最低,HTPKO中维生素E(4.67±0.03µg/ml)含量最高(p<0.05)。NTPKO的FRAP最高(780.00±1.82µg/100g), DPO的FRAP最低(601.30±0.91µg/100g) (p<0.05)。油品对DPPH自由基的抑制率呈浓度依赖性增加(p<0.05),抗氧化能力约为70-80%。从油样品的物理化学和抗氧化特性中获得的结果显示出显著的积极作用,从而证明了其民族医学用途。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Assessment of the Phytochemical Constituents and Antioxidant Properties of Oils from Dura and Tenera Varieties of Elaeis guineensis Fruits
There have been acclaimed medicinal benefits of palm oil and palm kernel oil by rural dwellers in the southeastern part of Nigeria. The study investigated the phytochemical constituents and antioxidant properties of the different varieties of the oil using standard methods. The oil samples include: DPO (dura palm oil), TPO (tenera palm oil), NDPKO (dura native palm kernel oil), NTPKO (native tenera palm kernel oil), HDPKO (dura palm kernel oil of hexane extract) and HTPKO (tenera palm kernel oil of hexane extract). Results obtained revealed the presence of steroids, glycosides, terpenoids, triterpenes and phenolics in all the oil samples whereas saponins, flavonoids and coumarins were only detected in DPO, TPO and NDPKO. The result of the quantitative phytochemical analysis of the oil samples showed that NDPKO statistically had the highest contents of terpenoids (1.77 ± 0.14 mg/kg) and triterpenes (288.50 ± 23.30 mg/100g). Saponins, flavonoids and coumarins were highest in DPO with values corresponding to 1.00 ± 0.001 mg/kg, 308.30 ± 1.81 mg/100g and 43.98 ± 0.13 µg/100g respectively. Furthermore, NTPKO was found to have the highest content of steroids (150.40 ± 0.24 mg/100g) while TPO had the highest content of glycosides (9.01 ± 0.07 mg/100g) and phenolics (45.04 ± 0.12 mg/100g) at p<0.05. TPO had the highest content of β-carotene (2.06 ± 0.00 mg/100ml) and vitamin A (105.60 ± 0.15 IU/100ml) but had the lowest vitamin E content (3.73± 0.30 µg/ml) whereas HTPKO had the highest (p<0.05) content of vitamin E (4.67 ± 0.03 µg/ml) among the oil samples. NTPKO had the highest FRAP (780.00 ± 1.82 µg/100g) while DPO had the least (p<0.05) FRAP (601.30 ± 0.91 µg/100g). The oil samples exhibited a concentration-dependent increase (p<0.05) in percentage inhibition of DPPH free radical showing an antioxidant capacity of about 70-80%. The results obtained from the physicochemical and antioxidant properties of the oil samples showed significant positive effects thus justifying their ethnomedical use.
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