光活化光对紫外线辐射引起的人体皮肤变化的影响

S. E. Whitmore, C. Potten, C. Chadwick, P. Strickland, W. Morison
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引用次数: 14

摘要

背景/目的:紫外线照射(UVR)后的光再活化光(PRL)通过激活光解酶引起环丁烷嘧啶二聚体的光逆转。虽然在“生命三国”中已经证明了光逆转,但它在人类中的存在仍然存在争议。我们在人类身上寻找光反转的证据。方法与结果:7名受试者在2个部位接受4个最小红斑剂量(MED)的太阳模拟紫外线照射。在这两个地点中,其中一个随即暴露于PRL源。暴露后立即进行表皮活组织检查。与“UVR, prl暴露”位点相比,“仅UVR”位点与“UVR, prl暴露”位点在嘧啶二聚体数量上没有显著差异。24 h后复查活检,p53蛋白表达及树突状细胞数量无明显差异。然而,“UVR, prl暴露”位点的嘧啶二聚体数量减少幅度更大。结论:我们没有发现证据表明PRL在人体中引起uvr诱导的嘧啶二聚体光逆转的直接作用。然而,我们的研究结果确实表明,PRL的一些间接作用可能会增强UVR暴露后24小时内嘧啶二聚体的修复。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of photoreactivating light on UV radiation-induced alterations in human skin
Background/Aims: Photoreactivating light (PRL) after ultraviolet radiation (UVR) exposure causes photoreversal of cyclobutane pyrimidine dimers through the activation of photolyase. Although photoreversal has been demonstrated in the ‘three kingdoms of life,’ its existence in man remains controversial. We sought evidence for photoreversal in man. Methods and Results: Seven subjects were spot-irradiated at two sites with 4 minimal erythema doses (MED) of solar-simulating UVR. Of the two sites, one was then immediately exposed to a PRL source. Epidermal biopsies were taken immediately after exposure. No significant difference in the quantity of pyrimidine dimers was detected comparing the ‘UVR only’ site to the ‘UVR, PRL-exposed’ site. Biopsies were repeated 24 h later and no significant difference in p53 protein expression or dendritic cell number was detected. However, the ‘UVR, PRL-exposed’ site showed a greater reduction in pyrimidine dimer quantity. Conclusions: We found no evidence for a direct effect of PRL causing photoreversal of UVR-induced pyrimidine dimers in man. Our results do, however, suggest that some indirect effect of PRL may enhance pyrimidine dimer repair in the 24-h period following UVR exposure.
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