{"title":"乙二胺铂(II)或三胺铂(II)与胸苷的结合位点和平衡常数","authors":"Kenji Inagaki, Yoshinori Kidani","doi":"10.1016/S0006-3061(00)80027-7","DOIUrl":null,"url":null,"abstract":"<div><p>The complex of thymidine with (NH<sub>3</sub>)<sub>3</sub>Pt(OH<sub>2</sub>) or (en)Pt(OH<sub>2</sub>)<sub>2</sub>, where en = ethylenediamine, have been prepared in aqueous solution at pH = 7. The isolated complexes, corresponding to the formula [(NH<sub>3</sub>)<sub>3</sub>Pt(ThdH<sub>−1</sub>)]NO<sub>3</sub> and (en)Pt(ThdH<sub>−1</sub>)<sub>2</sub> where ThdH<sub>−1</sub> = mono anion of thymidine, have been characterized by nmr, ir, and uv spectra. The binding site is the N(3) position of ThdH<sub>−1</sub>.</p><p>The equilibrium constant for (NH<sub>3</sub>)<sub>3</sub>Pt(OH<sub>2</sub>) + ThdH<sub>−1</sub> ⇌ (NH<sub>3</sub>)<sub>3</sub>Pt(ThdH<sub>−1</sub>), in which the equilibrium of binding is pH dependent, was estimated from integrated intensity ratio of the proton of position 6 of thymidine, and log <em>K</em> was 10.4 ± 0.1. In the case of (en)Pt(ThdH<sub>−1</sub>)<sub>2</sub>, the log <em>K</em><sub>1</sub> for (en)Pt(OH<sub>2</sub>)<sub>2</sub> + ThdH<sub>−1</sub> ⇌ (en)Pt(OH<sub>2</sub>)(ThdH<sub>−1</sub>) was 10.3 ± 0.1, and the log <em>K</em><sub>2</sub> for (en)Pt(OH<sub>2</sub>)(ThdH<sub>−1</sub>) + ThdH<sub>−1</sub> ⇌ (en)Pt(ThdH<sub>−1</sub>)<sub>2</sub> was 7.4 ± 0.1.</p></div>","PeriodicalId":9177,"journal":{"name":"Bioinorganic chemistry","volume":"9 4","pages":"Pages 333-343"},"PeriodicalIF":0.0000,"publicationDate":"1978-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0006-3061(00)80027-7","citationCount":"4","resultStr":"{\"title\":\"Binding site and equilibrium constant of ethylenediamineplatinum(II) or triamineplatinum(II) to thymidine\",\"authors\":\"Kenji Inagaki, Yoshinori Kidani\",\"doi\":\"10.1016/S0006-3061(00)80027-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The complex of thymidine with (NH<sub>3</sub>)<sub>3</sub>Pt(OH<sub>2</sub>) or (en)Pt(OH<sub>2</sub>)<sub>2</sub>, where en = ethylenediamine, have been prepared in aqueous solution at pH = 7. The isolated complexes, corresponding to the formula [(NH<sub>3</sub>)<sub>3</sub>Pt(ThdH<sub>−1</sub>)]NO<sub>3</sub> and (en)Pt(ThdH<sub>−1</sub>)<sub>2</sub> where ThdH<sub>−1</sub> = mono anion of thymidine, have been characterized by nmr, ir, and uv spectra. The binding site is the N(3) position of ThdH<sub>−1</sub>.</p><p>The equilibrium constant for (NH<sub>3</sub>)<sub>3</sub>Pt(OH<sub>2</sub>) + ThdH<sub>−1</sub> ⇌ (NH<sub>3</sub>)<sub>3</sub>Pt(ThdH<sub>−1</sub>), in which the equilibrium of binding is pH dependent, was estimated from integrated intensity ratio of the proton of position 6 of thymidine, and log <em>K</em> was 10.4 ± 0.1. In the case of (en)Pt(ThdH<sub>−1</sub>)<sub>2</sub>, the log <em>K</em><sub>1</sub> for (en)Pt(OH<sub>2</sub>)<sub>2</sub> + ThdH<sub>−1</sub> ⇌ (en)Pt(OH<sub>2</sub>)(ThdH<sub>−1</sub>) was 10.3 ± 0.1, and the log <em>K</em><sub>2</sub> for (en)Pt(OH<sub>2</sub>)(ThdH<sub>−1</sub>) + ThdH<sub>−1</sub> ⇌ (en)Pt(ThdH<sub>−1</sub>)<sub>2</sub> was 7.4 ± 0.1.</p></div>\",\"PeriodicalId\":9177,\"journal\":{\"name\":\"Bioinorganic chemistry\",\"volume\":\"9 4\",\"pages\":\"Pages 333-343\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1978-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0006-3061(00)80027-7\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioinorganic chemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0006306100800277\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioinorganic chemistry","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0006306100800277","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Binding site and equilibrium constant of ethylenediamineplatinum(II) or triamineplatinum(II) to thymidine
The complex of thymidine with (NH3)3Pt(OH2) or (en)Pt(OH2)2, where en = ethylenediamine, have been prepared in aqueous solution at pH = 7. The isolated complexes, corresponding to the formula [(NH3)3Pt(ThdH−1)]NO3 and (en)Pt(ThdH−1)2 where ThdH−1 = mono anion of thymidine, have been characterized by nmr, ir, and uv spectra. The binding site is the N(3) position of ThdH−1.
The equilibrium constant for (NH3)3Pt(OH2) + ThdH−1 ⇌ (NH3)3Pt(ThdH−1), in which the equilibrium of binding is pH dependent, was estimated from integrated intensity ratio of the proton of position 6 of thymidine, and log K was 10.4 ± 0.1. In the case of (en)Pt(ThdH−1)2, the log K1 for (en)Pt(OH2)2 + ThdH−1 ⇌ (en)Pt(OH2)(ThdH−1) was 10.3 ± 0.1, and the log K2 for (en)Pt(OH2)(ThdH−1) + ThdH−1 ⇌ (en)Pt(ThdH−1)2 was 7.4 ± 0.1.
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