卵母细胞和山羊卵泡输卵管表达特异性糖蛋白1 (OVGP1)的研究

A. Firmawati, Herawati, H. Pratiwi, L. ‘Ainiyah, A. S. R. A. Rozzaaq
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引用次数: 0

摘要

输卵管特异性糖蛋白(OVGP1)是一种糖蛋白,由山羊输卵管内的非纤毛分泌上皮细胞分泌,分子量为65 kDa。这种蛋白的表达非常依赖于雌激素水平和物种的发情期。另一方面,糖蛋白在卵母细胞成熟、精子头化和早期胚胎发育过程中起着重要作用。本研究的目的是寻找OVGP1在印尼内源山羊卡仓山羊(Capra hircus)卵母细胞和卵泡中的表达位置。本研究使用的方法是:免疫细胞化学技术观察OVGP1在卵母细胞和山羊卵泡中的表达。在山羊卵母细胞(Capra hircus)中,OVGP1在卵孔积云切片、透明带、卵泡周围间隙和卵浆膜中表达,而在卵泡中,OVGP1在颗粒细胞、外膜细胞和内膜细胞中表达。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Study of Oviduct Expression Specificct Glycoprotein1 (OVGP1) on Oocyte and Goat Follicles (Capra hircus)
: Oviduct Specific Glycoprotein (OVGP1) is a glycoprotein that has been identified as a protein secreted from unciliated secretory epithelial cells in oviducts with a molecular weight of 65 kDa in goats. The expression of this protein is very dependent on estrogen levels and the oestrus phase of the species. On the other hand, glycoprotein plays an important role in the process of oocyte maturation, spermatozoa capitation, and early embryonic development. The purpose of this research was to find the location of OVGP1 expression in oocytes and follicles of kacang goat (Capra hircus), an endogenous goat from Indonesian . The methods used in this study are: immunocytochemical techniques to see OVGP1 expression on oocytes and on goat follicles. OVGP1 expression in goat oocytes (Capra hircus) observed using immunocytochemical techniques was detected in the cumulus ooporus section, the zona pellucida, perivitelline space, and plasma oocyte membrane, whereas in the follicle the OVGP1 expression observed using immunohistochemical methods showed that OVGP1 was expressed in granulosa cells, external and internal theca cells.
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