受乳腺炎影响的泌乳奶牛中金黄色葡萄球菌和大肠杆菌的常用治疗抗菌药及其耐药性模式。

IF 4 3区 地球科学 Q2 ENGINEERING, ELECTRICAL & ELECTRONIC
IEEE Geoscience and Remote Sensing Letters Pub Date : 2022-02-28 eCollection Date: 2022-01-01 DOI:10.1080/23144599.2022.2038494
Eaftekhar Ahmed Rana, Md Abul Fazal, Mohammad Abdul Alim
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引用次数: 0

摘要

乳腺炎是奶牛生产中最常见、最昂贵的疾病之一。通常使用广谱抗菌药物进行治疗。这项工作的目的是调查金黄色葡萄球菌和大肠杆菌的流行情况,找出用于治疗乳腺炎的抗菌药物,并探索抗菌药物的耐药性概况,包括耐药基因的检测。细菌种类和抗菌药耐药性基因通过聚合酶链反应进行确认。共筛查了 450 头奶牛,其中 23 头(5.11%)和 173 头(38.44%)分别患有临床和亚临床乳腺炎。在临床和亚临床乳腺炎奶牛中,金黄色葡萄球菌的感染率分别为 39.13%(9 头)和 47.97%(83 头),而大肠杆菌的感染率分别为 30.43%(7 头)和 15.60%(27 头)。治疗乳腺炎使用最多的抗菌药是环丙沙星(83.34%)、阿莫西林(80%)和头孢曲松(76.67%)。超过 70% 的金黄色葡萄球菌对氨苄西林、氧西林和四环素产生耐药性,超过 60% 的大肠杆菌对氧西林和磺胺甲噁唑-三甲氧苄青霉素产生耐药性。从金黄色葡萄球菌和大肠杆菌中发现了部分抗菌药耐药基因(mecA、tetK、tetL、tetM、tetA、tetB、tetC、sul1、sul2 和 sul3)。令人惊讶的是,7 个(7.61%)金黄色葡萄球菌携带 mecA 基因,并被确认为耐甲氧西林金黄色葡萄球菌(MRSA)。金黄色葡萄球菌最常见的耐药基因是 tetK 18(19.57%)和 tetL 13(14.13%),而大肠杆菌最常见的耐药基因是 sul1 16(47.06%)、tetA 12(35.29%)、sul2 11(32.35%)和 tetB 7(20.59%)。抗菌素的滥用和耐多药细菌的存在对公众健康构成了潜在威胁。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Frequently used therapeutic antimicrobials and their resistance patterns on Staphylococcus aureus and Escherichia coli in mastitis affected lactating cows.

Mastitis is one of the most frequent and costly production diseases of dairy cattle. It is frequently treated with broad-spectrum antimicrobials. The objectives of this work were to investigate the prevalence of Staphylococcus aureus and Escherichia coli, find out the antimicrobials used in mastitis treatment, and explore the antimicrobial resistance profile including detection of resistance genes. Bacterial species and antimicrobial resistance genes were confirmed by the polymerase-chain reaction. A total of 450 cows were screened, where 23 (5.11%) and 173 (38.44%) were affected with clinical and sub-clinical mastitis, respectively. The prevalence of S. aureus was 39.13% (n = 9) and 47.97%(n = 83) while, E. coli was 30.43% (n = 7) and 15.60% (n = 27) in clinical and sub-clinical mastitis affected cows, respectively. The highest antimicrobials used for mastitis treatment were ciprofloxacin (83.34%), amoxycillin (80%) and ceftriaxone (76.67%). More than, 70% of S. aureus showed resistance against ampicillin, oxacillin, and tetracycline and more than 60% of E. coli exhibited resistance against oxacillin and sulfamethoxazole-trimethoprim. Selected antimicrobial resistance genes (mecA, tetK, tetL, tetM, tetA, tetB, tetC, sul1, sul2 and sul3) were identified from S. aureus and E. coli. Surprisingly, 7 (7.61%) S. aureus carried the mecA gene and were confirmed as methicillin-resistant S. aureus (MRSA). The most prevalent resistance genes were tetK 18 (19.57%) and tetL 13 (14.13%) for S. aureus, whereas sul1 16 (47.06%), tetA 12 (35.29%), sul2 11 (32.35%) and tetB 7 (20.59%) were the most common resistance genes in E. coli. Indiscriminate use of antimicrobials and the presence of multidrug-resistant bacteria suggest a potential threat to public health.

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来源期刊
IEEE Geoscience and Remote Sensing Letters
IEEE Geoscience and Remote Sensing Letters 工程技术-地球化学与地球物理
CiteScore
7.60
自引率
12.50%
发文量
1113
审稿时长
3.4 months
期刊介绍: IEEE Geoscience and Remote Sensing Letters (GRSL) is a monthly publication for short papers (maximum length 5 pages) addressing new ideas and formative concepts in remote sensing as well as important new and timely results and concepts. Papers should relate to the theory, concepts and techniques of science and engineering as applied to sensing the earth, oceans, atmosphere, and space, and the processing, interpretation, and dissemination of this information. The technical content of papers must be both new and significant. Experimental data must be complete and include sufficient description of experimental apparatus, methods, and relevant experimental conditions. GRSL encourages the incorporation of "extended objects" or "multimedia" such as animations to enhance the shorter papers.
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