单镜头三维荧光显微镜与傅里叶扩散凸轮

Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP) Pub Date : 2019-04-14 DOI:10.1364/NTM.2019.NS2B.3

F. Liu, Vaishnavi Madhavan, N. Antipa, Grace Kuo, Saul Kato, L. Waller

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引用次数: 9

摘要

我们提出了一种单镜头三维荧光显微镜，实现了大视野和良好的分辨率跨宽轴向范围。这是通过插入一个漫射器到目标的傅里叶平面和解决一个逆问题来实现的。我们展示了在25 fps下自由移动的秀丽隐杆线虫的实验3D结果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Single-shot 3D fluorescence microscopy with Fourier DiffuserCam

We propose a single-shot 3D fluorescence microscope that achieves large field-of-view and good resolution across a wide axial range. This is implemented by inserting a diffuser into the Fourier plane of the objective and solving an inverse problem. We show experimental 3D results of a freely-moving C. elegans nematode at 25 fps.

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来源期刊

Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)

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