Karakterisasi dan Penetapan Kadar Flavonoid Total Ekstrak dan Fraksi Bunga Pepaya Jantan (Carica papaya L.) dengan Spektrofotometri UV-Vis

AbstractBackground: The potential of male papaya flowers (Carica papaya L.) as functional herbal ingredients, such as antioxidants, is influenced by the activity of secondary metabolites, one of them is flavonoid.Objective: This study to determine the content of flavonoid and characterization flavonoids in ethanol extract and fractions of male papaya flower (Carica papaya L.).Method: Male papaya flower was maceration using ethanol 70% then fractionated using multilevel fractionation. Identification flavonoid was tested by using the Taubeck, Wilstater, and TLC. Extract and fractions were determined the content of flavonoid using colorimetric method and identification of flavonoids based on the wavelength of cinnamoyl and benzoyl in the spectrum UV-Vis.Results: The results of the determination of flavonoid content showed that ethanol extract was the highest 0.6805 ± 0.0045 %QE and n-hexane fractions was the lowest 0.4178 ± 0.0058 %QE. Identification of flavonoids using UV-Vis spectroscopy method showed the absorption of substituted 3-OH flavonol and free 3-OH flavonol.Conclusion: The highest flavonoid content was found in the ethanol extract of male papaya flower. The types of flavonoids in the extract and fractions of male papaya flower indicated the type of flavonol based on the wavelength of cinnamoyl and benzoyl.Keywords: carica, flavonoid, cinnamoyl, benzoyl, UV-Vis Intisari Latar belakang: Potensi bunga pepaya jantan (Carica papaya L.) sebagai bahan fungsional herbal, seperti antioksidan dipengaruhi oleh aktivitas dari metabolit sekunder, salah satunya flavonoid.Tujuan: Penelitian ini bertujuan untuk mengetahui kadar flavonoid total serta karakterisasi flavonoid dalam ekstrak etanol dan fraksi bunga pepaya jantan (Carica papaya L.).Metode: Bunga pepaya jantan dilakukan maserasi dengan etanol 70%, dilanjutkan fraksinasi bertingkat. Identifikasi flavonoid dilakukan menggunakan metode Taubeck, Wilstater dan KLT. Kadar flavonoid ekstrak dan fraksi ditetapkan menggunakan metode kolorimetri serta identifikasi flavonoid berdasarkan pita cinnamoyl dan benzoyl pada spektrum UV-Vis.Hasil: Hasil penetapan kadar flavonoid menunjukkan bahwa ekstrak etanol memiliki kadar tertinggi sebesar 0,6805 ± 0,0045 %QE, serta kadar terendah dalam fraksi n-heksan sebesar 0,4178 ± 0,0058 %QE. Identifikasi flavonoid metode spektrofotometri UV-Vis menunjukkan penyerapan flavonol 3-OH substitusi dan flavonol 3-OH bebas.Kesimpulan: Kadar flavonoid tertinggi ditemukan dalam ekstrak etanol bunga pepaya. Jenis flavonoid dalam ekstrak dan fraksi bunga pepaya jantan mengindikasikan jenis flavonol berdasarkan panjang gelombang dari cinnamoyl dan benzoyl.Kata kunci : carica, flavonoid, cinnamoyl, benzoyl, UV-Vis