菊叶提取物对实验动物的抗糖尿病作用评价

YMER Digital Pub Date : 2022-08-17 DOI:10.37896/ymer21.08/54
A. Verma
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引用次数: 0

摘要

糖尿病(DM)是一种长期疾病,可由胰腺胰岛素分泌不足或胰岛素分泌无效引起。在本研究中,分别以100、200、400 mg/kg剂量的甲醇、50% v/v的氢酒精甲醇和牛蒡叶提取物为口服剂量,以50 mg/kg剂量的二甲双胍为单早晨剂量,连续给药21 d,观察其抗糖尿病作用。在血糖正常的大鼠中,禁食18小时评估药物的抗糖尿病作用。在实验第0、10、21天,采用自动分析仪采集葡萄糖氧化酶法读数,评估空腹血糖水平。测定抗氧化指标包括:总酚含量、总黄酮浓度、DPPH自由基清除能力、羟基自由基清除能力、过氧化氢清除能力、超氧化物自由基清除能力、一氧化氮清除能力和还原能力。菊叶甲醇(ICME)和水醇(ICHE)提取物(100、200、400 mg/kg, p.o)通过口服葡萄糖耐量试验。两种提取物在降低血糖水平方面都有更大的作用。其中,与对照组相比,ICHE在所有剂量下均显示显著效应(P<0.05-P<0.001)。在葡萄糖耐量方面,ICME在所有剂量下都有显著的效果。关键词:糖尿病;胰岛素;二甲双胍;
本文章由计算机程序翻译,如有差异,请以英文原文为准。
ASSESSMENT OF INULA CAPPA LEAVES EXTRACTS FOR ANTIDIABETIC POTENTIAL IN EXPERIMENTAL ANIMALS
Diabetes mellitus (DM) is a long-term disease that can be caused by a lack of insulin production by the pancreas, or by the ineffectiveness of the insulin produced. For the study, the antidiabetic effects of methanolic and 50% v/v hydroalcoholic methanolic and extracts of the leaves of Inula cappa were used as oral doses at 100, 200, and 400 mg/kg and Metformine (50 mg/kg) were administered continuously for 21 days as a single morning dose. 18 hours of fasting was used to assess the drug's anti-diabetic effects in normoglycemic rats. On the 0th, 10th, and 21st days of the experiment, glucose oxidase method readings were collected using an autoanalyzer to assess fasting blood glucose levels. Anti-oxidant parameters were determined including, total phenolic content, total flavonoid concentration, DPPH free radical scavenging activity, Hydroxyl radical scavenging activity, Scavenging of Hydrogen Peroxide, Superoxide radical scavenging assay, Nitric oxide scavenging activity and Reducing power assay. The methanolic (ICME) and hydroalcoholic (ICHE) extracts of Inula cappa leaves (100, 200, and 400 mg/kg, p.o.) passes the Oral Glucose Tolerance Test. Both extracts had a greater impact in lowering blood glucose levels. Out of which ICHE at all doses showed significant effect (P<0.05-P<0.001) when compared with control. ICME in terms of glucose tolerance at all doses had significant effects. Keywords: Diabetes mellitus, insulin, metformine, scavenging, Inula cappa.
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