辣椒素纳米颗粒对体外肝细胞癌的抗癌作用

N. Hazem, W. Elkashef, I. El-Sherbiny, A. Emam, D. Shaalan, M. Sobh
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引用次数: 4

摘要

肝细胞癌(HCC)是世界上第五大最常见的癌症,由于高转移和复发率,其总生存率较低。本研究的目的是评估和比较辣椒素和纳米配方辣椒素在体外肝癌人细胞系HepG2中可能的抗肿瘤作用。细胞系的来源,包括获得的时间和地点。细胞系最近是否经过鉴定,用什么方法鉴定。该细胞系最近是否检测过支原体污染。采用阳离子三甲基壳聚糖与辣椒素离子化凝胶法制备了辣椒素负载三甲基壳聚糖纳米颗粒。通过TEM和zeta分析仪对合成的纳米颗粒进行了表征。培养人肝癌HepG2细胞,分别用50、75和100 μM辣椒素(CAP)、普通TMCS NPs和cl -NPs以及乙醇(对照)处理24h和48h。采用流式细胞术、免疫细胞化学检测Bcl-2、Bax和caspase蛋白,实时荧光定量PCR检测Bcl-2、Bax和MDR-1 mRNA基因表达水平。我们的研究结果表明,与普通辣椒素制剂相比,辣椒素负载的NPs有可能通过改变凋亡调节因子的表达或改变MDR-1的表达来诱导凋亡,从而显著提高辣椒素的生物活性。本研究所研究的tmc纳米颗粒可能是一种很好的辣椒素给药载体。在HCC治疗中应用辣椒素负载NPs作为一种辅助治疗方法,可能是提高常规化疗的治疗效果和耐药性的一种新策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Anticarcinogenic Effects of Capsaicin-Loaded Nanoparticles on In vitro Hepatocellular Carcinoma
Hepatocellular Carcinoma (HCC) is the fifth most frequent cancer worldwide with a low overall survival due to high metastasis and recurrence rates. The aim of this study is to assess and compare the possible anti-neoplastic effect of capsaicin and nanoformulated capsaicin on in vitro HCC human cell line HepG2. The source of the cell line, including when and from where it was obtained. Whether the cell line has recently been authenticated and by what method. Whether the cell line has recently been tested for mycoplasma contamination. Capsaicin-loaded Trimethyl Chitosan Nanoparticles (CL TMCS NPs) were synthesized by ionotropic gelation of cationic TMCS with capsaicin. The synthesized nanoparticles were characterized through TEM, and zeta analyzer. Human hepatocarcinoma HepG2 cell lines were cultured and treated with 50, 75 & 100 μM of Capsaicin (CAP), plain TMCS NPs and CL-NPs as well as ethanol (control) for 24h and 48h. The induced effects were investigated by flow cytometry, immunocytochemistry assay for Bcl-2, Bax, and caspase proteins and evaluating gene expression levels of Bcl-2, Bax, and MDR-1 mRNA by real-time PCR. Our results demonstrated that capsaicin- loaded NPs had the potential to significantly increase capsaicin bioactivity compared with the plain capsaicin formulation either in inducing apoptosis through altering expression of apoptotic regulators or modifying MDR-1 expression. TMCs nanoparticles investigated in this study may be a good drug delivery vehicle for capsaicin. Application of capsaicin-loaded NPs in HCC management as an adjunct therapeutic approach may be a novel strategy to improve the treatment efficacy and resistance of the conventionally used chemotherapy.
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来源期刊
Current Chemical Biology
Current Chemical Biology Medicine-Biochemistry (medical)
CiteScore
1.40
自引率
0.00%
发文量
16
期刊介绍: Current Chemical Biology aims to publish full-length and mini reviews on exciting new developments at the chemistry-biology interface, covering topics relating to Chemical Synthesis, Science at Chemistry-Biology Interface and Chemical Mechanisms of Biological Systems. Current Chemical Biology covers the following areas: Chemical Synthesis (Syntheses of biologically important macromolecules including proteins, polypeptides, oligonucleotides, oligosaccharides etc.; Asymmetric synthesis; Combinatorial synthesis; Diversity-oriented synthesis; Template-directed synthesis; Biomimetic synthesis; Solid phase biomolecular synthesis; Synthesis of small biomolecules: amino acids, peptides, lipids, carbohydrates and nucleosides; and Natural product synthesis).
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