枸杞种子精油纳米乳(lsei - ne)对金黄色葡萄球菌的抗氧化及杀菌活性研究

Roghaye Keykhasalar, Masoud Homayouni Tabrizi, P. Ardalan
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引用次数: 8

摘要

背景:紫草种子精油(LSEO)是一种有效的抗菌化合物,含有多种植物化学物质,如木脂素和酚类。目的:制备LSEO纳米乳(LSEO- ne),研究其抗氧化能力和对金黄色葡萄球菌的杀菌活性。方法:采用超声法制备lsei - ne,并采用原子力显微镜(AFM)、动态光散射(DLS)和扫描电镜(SEM)对其进行表征。然后,与谷胱甘肽相比,我们利用ABTS和DPPH测试在四种不同的LSEO-NE浓度(200、600、800和1000µg/mL)下测量其抗氧化活性。最后,我们通过比较lseo - ne涂片与未涂片和卡那霉素涂片的抗生素药敏试验(AST)来评估其对金黄色葡萄球菌的杀菌活性。结果:PDI为0.452的67.3 nm lsio2 - ne液滴在ABTS (IC50 = 350µg/mL)和DPPH (IC50 = 235µg/mL)试验中均表现出与谷胱甘肽相似的抗氧化活性。此外,AST结果显示金黄色葡萄球菌对lseo - ne涂片的椎间盘与未涂片和卡那霉素的椎间盘相比具有显著的敏感性。结论:lsei - ne可作为一种安全、天然、有效的抗生素用于大多数器官,如呼吸系统和皮肤,金黄色葡萄球菌引起的细菌感染。然而,需要进一步的体内研究来评估LSEO-NE对其他致病性金黄色葡萄球菌菌株的抗菌效果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antioxidant Property and Bactericidal Activity of Linum usitatissimum Seed Essential Oil Nanoemulsion (LSEO-NE) on Staphylococcus aureus
Background: Linum usitatissimum Seed Essential Oil (LSEO) as an efficient antimicrobial compound contains various types of phytochemicals, such as lignans and phenols. Objectives: In the current study, we produced LSEO nanoemulsion (LSEO-NE) to study its antioxidant capacity and bactericidal activity against Staphylococcus aureus. Methods: The LSEO-NE was produced using the ultrasonication method and characterized by Atomic Force Microscopy (AFM), Dynamic Light Scattering (DLS), and Scanning Electron Microscopy (SEM). Then, we measured its antioxidant activity utilizing both ABTS and DPPH tests at four different LSEO-NE concentrations (200, 600, 800, and 1000 µg/mL) compared to glutathione. Finally, we evaluated its bactericidal activity on S. aureus by studying Antibiotic Susceptibility Testing (AST) using LSEO-NE-smeared discs compared to non-smeared and kanamycin discs. Results: The 67.3 nm droplets of LSEO-NE with PDI of 0.452 exhibited strong antioxidant activity, similar to glutathione, in both ABTS (IC50 = 350 µg/mL) and DPPH (IC50 = 235 µg/mL) tests. Moreover, the AST results revealed the significant sensitivity of S. aureus to LSEO-NE-smeared discs when compared to non-smeared and kanamycin discs. Conclusions: According to the results, LSEO-NE can be applied as a safe, natural, and effective antibiotic for bacterial infections caused by S. aureus in most organs, such as the respiratory system and skin. However, further in vivo studies are required to evaluate the LSEO-NE antibacterial efficiency against other pathogenic S. aureus strains.
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